Influence of external dryness on defend barrier-cilium-mucous blanket in mouse airway
- VernacularTitle:外燥对小鼠气道"纤毛-黏液毯"御邪屏障影响的实验研究
- Author:
Jian-Zhong DING
1
;
Li-Qun HU
;
Liu-Tong ZHANG
;
Chang-Fu WANG
;
Shang-Rong HE
;
Ming-Ming WANG
Author Information
1. 长江大学
- Keywords:
external dryness;
cilium-mucous blanket;
secretory IgA;
mice
- From:
Journal of Beijing University of Traditional Chinese Medicine
2010;33(1):20-22,40
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe dynamically the influence of external dryness on the local defend barrier in mouse airway, and study the pathogenic mechanism.Method Special pathogenic free (SPF) Kunming mice (n=120) were randomly divided into the normal-temperature and normal-dampness group (group A), normal-temperature and dryness group (group B), warm-dryness group (group C) and cool-dryness group (group D, each n=30). The mouse model was established by the synthetic stimulation of imitated external dryness of temperature-relevant humidity-wind in the intelligent artificial climate box. After 6, 12 and 18 days, the histopathological changes of respiratory tract, and the content of total protein(P), mucopolysaccharide (MS) and secretory IgA (SIgA) in the fluid of respiratory tract were detected respectively.Result The histopathological changes in airway was not obvious in group B. In group C and D there were squamous metaplasia of airway epithelium and cilium function defect, and epithelial mucous metaplasia of 20% to 40% trachea serous gland accompanied by the infiltration of inflammatory cells from the 6~(th) day to the 18~(th) day, which were the most serious on the 12~(th) day. The content of P decreased from the 12~(th) day to the 18~(th) day in group B, C and D, content of MS tended to increase in group B, and content of MS and SIgA decreased continuously in group C (P<0.05). The content of P decreased continuously in group D from the 12~(th) day to the 18~(th) day (P<0.05) and the content of MS decreased on the 12th day (P<0.05).Conclusion The result reconfirms TCM doctrine of warm-dryness inducing the impairment of lung, and the decrease of SIgA content aggravates the pathological changes. One of the basic pathogeneses of external dryness is related to the attenuation of cilium-mucous blanket and local defend barrier. The result also indicates that the obvious airway pathological changes on the 12~(th) day provides a base of the best observation phase for the research on the pathogenesis of external dryness.
