Influence of therapy of regulating qi and activating blood circulation on gene expression profile in rats with precancerosis of atrophic gastritis
10.3321/j.issn:1006-2157.2009.12.010
- VernacularTitle:理气活血法对萎缩性胃炎癌前病变大鼠基因表达谱的影响
- Author:
Ning-Ning ZHAO
1
;
Jun-Xiang LI
;
Yu-Lu ZHANG
;
Rui SHI
;
Ling-Qun ZHU
Author Information
1. 北京中医药大学东方医院
- Keywords:
therapy of regulating qi and activating blood circulation;
atrophic gastritis;
precancerosis;
gene chip;
gene identification;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2009;32(12):830-833,后插2
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of TCM therapy of regulating qi and activating blood circulation on the gene expression profile of gastric mucosa in rats with precancerosis of atrophic gastritis.Methods The rat model of preeancerosis of atrophic gastritis was established by the synthetic method of metal spring insertion and feeding of NaCI solution and heat paste.The changes of differential gene expressions in the rats were detected by using gene chip technology after the intervention of the TCM therapy.The differential expression genes were screened and then identified by applying RT-PCR.Results There were totally 14 differential expression genes screened,and among them 8 were upregulated genes and 6 wero down-regulated genes.All of them consisted of the differential expression gene profde after the intervention of the TCM therapy in rat with precancerosis of atrophic gastritis,among which ptk2 was regulated significantly downward in the spontaneous recovery group and regulated significantly upward after the intervention of the TCM therapy.Conclusion The TCM therapy of regulating qi and activating blood circulation can regulate upward the gene expression of ptk2 in order to regulate the remodeling ofactin cytoskeleton,and improve the repairing and healing of gastric mucosa.It is may be one of the mechanisms of the TCM therapy for treating precancerosis of atrophic gastritis.
