Inhibitory effect of rhynchophylline on carotid sinus baroreflex in anesthetized rats
10.3321/j.issn:1006-2157.2009.11.011
- VernacularTitle:钩藤碱抑制麻醉大鼠颈动脉窦压力感受性反射的研究
- Author:
Yi-Xian LIU
1
;
Hui-Juan MA
;
Lu GAO
;
Zhi WEN
;
Na LIU
;
Yi ZHANG
;
Rui-Rong HE
Author Information
1. 河北医科大学
- Keywords:
rhynchophylline;
arterial baroreflex;
nitric oxide synthase inhibitor;
tetraethylamonium;
agonist of L-type calcium channel;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2009;32(11):763-767
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of rhynchophylline (Rhy) on carotid sinus baroreflex (CSB) and the mechanism.Methods The technique of carotid sinus isolation and perfusion was used to determine the parameters of CSB, including threshold pressure (TP), saturation pressure (SP), equilibrium pressure (EP), operating range (OR), peak slope (PS) and reflex decrease (RD). The influence of Rhy on CSB was observed in a slope manner after Rhy (10, 50 and 100 μmol/L) dissolving in K-H solution and then filling into carotid sinus. Nitric oxide synthase inhibitor[N~G-nitro-arginine methyl ester(L-NAME)], potassium channel blocker [tetraethylammonium (TEA)] and agonist of L-type calcium channel (Bay K8644) were perfused into isolated carotid sinus before reperfusion of Rhy, and then the effects of them on Rhy were observed. Results Rhy inhibited CSB in a manner of dose-dependent with the increase of TP and SP and the decrease of PS and RD. The inhibitory effect of Rhy was not affected by the pretreatment of L-NAME (300 μmol/L) or TEA (1 mmol/L), but attenuated by Bay K8644 (500 nmol/L).Conclusion Rhy inhibits CSB, which may be related to that Rhy attenuates calcium influx of mechanosensitive ion channel of baroreceptor but not related to NO channel or potassium channel.
