Technique of bionic enzymatic method for extracting Scolopendra

VernacularTitle:仿生酶解法提取蜈蚣的工艺研究
Author: Neng-Ting HUANG ¹ ; Yu-Rong WANG ; Wen-Bo XU ; Shao-Hua ZHAO ; Yu-Jie ZHANG
Author Information

1. 北京中医药大学
Keywords: Scolopendra; bionic enzymatic method; active partial thromboplastin time; pepsin; trypsin; anti -coagulation
From: Journal of Beijing University of Traditional Chinese Medicine 2009;32(10):706-709
CountryChina
Language:Chinese
Abstract: Objective To study the technology condition of extracting Scolopendra by bionic enzymatic method. Methods The technology condition of extracting Scolopendra by bionic enzymatic method was observed by single factor observation taken the hydrolysis degree as the index, and the extractive technique was verified by using the methods of active partial thromboplastin time ( APTT) and fibrinogen plate. Results The coagulation time was longer and anti-thrombosis effect was more significant in the bionic enzymatic method. The best enzymatic condition was as follows; Scolopendra was hydrolyzed in artificial gastric juice ( 37 ℃ ) and pepsin ( ratio of pepsin to substrate was 4. 0% ) for 4 hours, and then the residue was hydrolyzed in artificial intestinal juice ( 37 ℃) and trypsin ( ratio of pepsin to substrate was 5%) for 4 hours. Conclusion The bionic enzymatic method can be used in the extraction of effective components of anti-coagulation and thrombolysis from Scolopendra.