Quantitative determination of the total saponins from Flos Hostae Plantagineae by ultraviolet-visible spectrophotometry

VernacularTitle:可见分光光度法测定玉簪花中总皂苷的含量
Author: Hong-Xia XIE ¹ ; Pei-Feng XUE ; Hong-Gui ZHANG ; Li-Qing FAN ; Hong-Mei BIAN
Author Information

1. 北京中医药大学
Keywords: Flos Hostae Plantagineae; gitogenin; total saponins; visible spectrophotometry; quantitative determination
From: Journal of Beijing University of Traditional Chinese Medicine 2009;32(9):624-625,645
CountryChina
Language:Chinese
Abstract: Objective To set up a ultraviolet-visible spectrophotometry for quantitative determination of the total saponins from Flos Hostae Plantagineae. Methods The ultraviolet-visible spectrophotometry was used to detect the absorbance with the wavelength of 450 nm taking vanillin-glacial acetic acid-concentrated sulphuric acid as the color-developing agent and gitogenin as the control sample. The content of the total saponins in the samples was calculated by using standard curve. Results Gitogenin showed a good linear relationship at range of 14～70 μg/ml (r=0.999 2) with the average recovery of 96.8% (RSD=2.97%, n=6). Conclusion The method is easy, accurate and sensitive with a higher repeatability, which can be used in the quantitative determination of the total saponins from Flos Hostae Plantagineae.