Influence of Dangguibuxue Decoction on protein expression of NF-κBp65 activated by ox-LDL in RAW264.7 cells
10.3321/j.issn:1006-2157.2009.09.008
- VernacularTitle:当归补血汤对ox-LDL激活RAW264.7细胞核转录因子-κBp65蛋白表达的影响
- Author:
Juan SUN
1
;
Shui-Qing HUANG
;
Wen-Jing MA
;
Lu SUN
;
Zhi-Qing LE
Author Information
1. 广州中医药大学
- Keywords:
Dangguibuxue Decoction;
RAW264.7 cells;
nuclear transcription factor-kappa Bp65;
oxidized low-density lipoprotein;
rabbits
- From:
Journal of Beijing University of Traditional Chinese Medicine
2009;32(9):608-610,648,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possibility and mechanism of Dangguibuxue Decoction in early prevention of the development of atherosclerosis (AS) based on the study of the effect of Dangguibuxue Decoction on the signal transduction iter of nuclear transcription factor-kappa Bp65 (NF-κBp65) in monocyte activated by oxidized low-density lipoprotein (ox-LDL) in RAW264.7 cells. Methods The rabbits were randomly divided into the control group and medicated serum group and then given respectively normal saline and Dangguibuxue Decoction orally for preparing the medicated serum. The suspension of RAW264.7 cells was vaccinated into culture flask and cultured for 24 hours, and then divided into the blank control group, ox-LDL group, inhibitor group and medicated serum group. Except the blank control group all other groups were given 100 mg/L ox-LDL to activate RAW264.7 cells. After incubation the cells were collected. The expression of NF-κBp65 was detected by using immunohistochemistry assay, and the quantity of activated protein in RAW264.7 cells was detected by using immunoblotting technique. Results In ox-LDL group there were a lot of brown-yellow positive granules observed in the cytochylema and nucleus of RAW264.7 cells, and less in the medicated serum group. The ratio of protein band gray scale was 1.288 3±0.013 9 in the ox-LDL group and 0.918 9±0.015 3 in the medicated serum group. There was a significant difference statistically between two groups (P<0.05). Conclusion Dangguibuxue Decoction can down-regulate the protein expression and activation of NF-κBp65 in RAW264.7 cells stimulated by ox-LDL. It may be one of the mechanisms of Dangguibuxue Decoction for antagonizing AS that the signal transduction iter of NF-κBp65 was blocked.
