Etiological characterization of invasive non-typhoid Salmonella strains in Guangdong Province from 2018 to 2022
10.3760/cma.j.cn112338-20230809-00060
- VernacularTitle:广东省2018-2022年侵袭性非伤寒沙门菌病原学特征分析
- Author:
Min ZOU
1
;
Dongmei HE
;
Jing XU
;
Qi CHENG
;
Fangzhu OUYANG
;
Leyan CHEN
;
Qifang CHEN
;
Changwen KE
;
Bixia KE
Author Information
1. 中山大学公共卫生学院，广州 510080
- Keywords:
Invasive non-typhoid Salmonella;
Serological identification;
Whole genome sequencing;
Drug resistance genes;
Virulence factor
- From:
Chinese Journal of Epidemiology
2024;45(4):520-528
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the serotype distribution, drug resistance and molecular characterization of invasive non-typhoid Salmonella (iNTS) in Guangdong Province from 2018 to 2022 and provide scientific evidence for the prevention and treatment of blood flow infection caused by Salmonella. Methods:Serological identification, antimicrobial susceptibility testing, multilocus sequence typing (MLST), and whole genome sequencing were performed on Salmonella isolated from blood and stool samples in Guangdong from 2018 to 2022. Simultaneously, annotated the sequencing results for drug resistance genes and virulence factors by a microbial gene annotation system. Results:The 136 iNTS strains were divided into 25 serotypes, and Salmonella enteritidis accounted for 38.24% (52/136). The OR of other iNTS serotypes were calculated with Salmonella typhimurium as the control. The OR values of Oreninburg, Rysson, and Pomona serotypes were the highest, which were 423.50, 352.92, and 211.75, respectively. The drug resistance rate of iNTS was 0.74%-66.91%, which was lower than that of non-iNTS (3.90%-77.21%). The main iNTS of drug resistance were ampicillin and tetracycline, with resistance rates of 66.91% (91/136) and 50.00% (68/136), respectively, while the resistance rates to ciprofloxacin (5.88%,8/136), ceftazidime (5.88%,8/136), gentamicin (5.13%,7/136) and cefoxitin (0.74%, 1/136) were relatively low. iNTS carried a variety of drug-resistance genes and virulence factors, but no standard virulence factor distribution has been found. MLST cluster analysis showed that iNTS was divided into 26 sequence types, and ST11 accounted for 38.24% (52/136). Conclusions:The iNTS strains in Guangdong were dominated by Salmonella enteritidis, of which three serotypes, Oreninburg, Rison, and Pomona, may be associated with a higher risk of invasive infection during 2018 to 2022 . iNTS was sensitive to clinical first-line therapeutic drugs (cephalosporins and fluoroquinolones), with highly diverse sequences and clear phylogenetic branches. ST11 was the local dominant clone group.
