DNA Methylation of KLRC1 and KLRC3 in Autoimmune Thyroiditis:Perspective of Different Water Iodine Exposure

Chen YAO; Liu JINJIN; Qu MENGYING; Ren BINGXUAN; Wu HUAIYONG; Zhang LI; Zhou ZHENG; Liu LIXIANG; Shen HONGMEI

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DNA Methylation of KLRC1 and KLRC3 in Autoimmune Thyroiditis:Perspective of Different Water Iodine Exposure

Author: Chen YAO ^{1
,

2
,

3} ; Liu JINJIN ; Qu MENGYING ; Ren BINGXUAN ; Wu HUAIYONG ; Zhang LI ; Zhou ZHENG ; Liu LIXIANG ; Shen HONGMEI
Author Information

1. Disorders Control,Centre for Endemic Disease Control,Chinese Centre for Disease Control and Prevention,Harbin Medical University,Harbin 150081,Heilongjiang,China
2. National Health Commission & Education Bureau of Heilongjiang Province,Key Laboratory of Etiology and Epidemiology,Harbin Medical University,Harbin 150081,Heilongjiang,China
3. Heilongjiang Provincial Key Laboratory of Trace Elements and Hum
Keywords: Autoimmune thyroiditis; DNA methylation; KLRC1; KLRC3; SH2D1B; Iodine
From: Biomedical and Environmental Sciences 2024;37(9):1044-1055
CountryChina
Language:Chinese
Abstract: Objective This study aimed to identify differentially methylated genes (DMGs) associated with natural killer cells in patients with autoimmune thyroiditis (AIT),focusing on the influence of varying water iodine exposure levels. Methods Participants were divided into categories based on median water iodine (MWI) concentrations:iodine-fortified areas (IFA,MWI<10 μg/L),iodine-adequate areas (IAA,40 ≤ MWI ≤ 100μg/L),and iodine-excessive areas (IEA,MWI>300 μg/L). A total of 176 matched AIT cases and controls were recruited and divided into 89,40,and 47 pairs for IFA,IAA,and IEA,respectively. DMGs were identified using 850K BeadChip analysis for 10/10 paired samples. Validation of DNA methylation and mRNA expression levels of the DMGs was conducted using MethylTarget? and QRT-PCR for 176/176 paired samples. Results KLRC1,KLRC3,and SH2D1B were identified as significant DMGs. Validation revealed that KLRC1 was hypomethylated and highly expressed,whereas KLRC3 was hypermethylated and highly expressed in individuals with AIT. Furthermore,KLRC1 was hypomethylated and highly expressed in both IFA and IEA. Conclusion The DNA methylation status of KLRC1 and KLRC3 may play crucial roles in AIT pathogenesis. Additionally,DNA methylation of KLRC1 seems to be influenced by different iodine concentrations in water.