Development of an Integrated Disposable Device for SARS-CoV-2 Nucleic Acid Extraction and Detection
- Author:
Ma JING
1
;
Hao YANZHE
;
Hou MEILING
;
Zhang XIAOSHAN
;
Liu JINGDUAN
;
Meng HAODI
;
Chang JIANGBO
;
Ma XUEJUN
;
Liu JIHUA
;
Ying QINGJIE
;
Wang XIANHUA
;
Li HONGXIA
;
Cao YUXI
;
Zhang XIAOGUANG
Author Information
- Keywords: An integrated disposable device; SARS-Cov-2; Nucleic acid detection
- From: Biomedical and Environmental Sciences 2024;37(6):639-646
- CountryChina
- Language:Chinese
- Abstract: Objective To develop a highly sensitive and rapid nucleic acid detection method for the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). Methods We designed,developed,and manufactured an integrated disposable device for SARS-CoV-2 nucleic acid extraction and detection.The precision of the liquid transfer and temperature control was tested.A comparison between our device and a commercial kit for SARS-Cov-2 nucleic acid extraction was performed using real-time fluorescence reverse transcription polymerase chain reaction(RT-PCR).The entire process,from SARS-CoV-2 nucleic acid extraction to amplification,was evaluated. Results The precision of the syringe transfer volume was 19.2±1.9 μL(set value was 20),32.2±1.6(set value was 30),and 57.2±3.5(set value was 60).Temperature control in the amplification tube was measured at 60.0±0.0 ℃(set value was 60)and 95.1±0.2 ℃(set value was 95)respectively.SARS-Cov-2 nucleic acid extraction yield through the device was 7.10×106 copies/mL,while a commercial kit yielded 2.98×106 copies/mL.The mean time to complete the entire assay,from SARS-CoV-2 nucleic acid extraction to amplification detection,was 36 min and 45 s.The detection limit for SARS-CoV-2 nucleic acid was 250 copies/mL. Conclusion The integrated disposable devices may be used for SARS-CoV-2 Point-of-Care test(POCT).
