Exposure Effects of Terahertz Waves on Primary Neurons and Neuron-like Cells Under Nonthermal Conditions
- Author:
Zhi Sheng TAN
1
;
Cheng Peng TAN
;
Qing Lan LUO
;
Liang Yun CHI
;
Long Zi YANG
;
Long Xue ZHAO
;
Li ZHAO
;
Ji DONG
;
Jing ZHANG
;
Wei Bin YAO
;
Ping Xin XU
;
Guang TIAN
;
Kui Jian CHEN
;
Hui WANG
;
Yun Rui PENG
Author Information
- Keywords: Terahertz wave; Biological effects; Neurons; Neurotransmitter; SYN; PSD95
- From: Biomedical and Environmental Sciences 2019;32(10):739-754
- CountryChina
- Language:Chinese
- Abstract: Objective This study aimed to explore the potential effects of terahertz (THz) waves on primary cultured neurons from 4 rat brain regions (hippocampus,cerebral cortex,cerebellum,and brainstem)and 3 kinds of neuron-like cells (MN9D,PC12,and HT22 cells) under nonthermal conditions.Methods THz waves with an output power of 50 (0.16 THz) and 10 (0.17 THz) mW with exposure times of 6 and 60 min were used in this study.Analysis of temperature change,neurite growth,cell membrane roughness,micromorphology,neurotransmitters and synaptic-related proteins (SYN and PSD95) was used to evaluate the potential effects.Results Temperature increase caused by the THz wave was negligible.THz waves induced significant neurotransmitter changes in primary hippocampal,cerebellar,and brainstem neurons and in MN9D and PC12 cells.THz wave downregulated SYN expression in primary hippocampal neurons and downregulated PSD95 expression in primary cortical neurons.Conclusion Different types of cells responded differently after THz wave exposure,and primary hippocampal and cortical neurons and MN9D cells were relatively sensitive to the THz waves.The biological effects were positively correlated with the exposure time of the THz waves.
