Molecular characterization of foodborne Yersinia enterocolitica strains in Liaocheng City, Shandong Province, from 2020 to 2021
10.3760/cma.j.cn112338-20220720-00645
- VernacularTitle:山东省聊城市2020-2021年小肠结肠炎耶尔森菌食品分离株分子特征分析
- Author:
Lu QIAN
1
;
Shengnan LIANG
;
Fangyuan CUI
;
Lihong CHENG
;
Jiangshen WANG
;
Ningning JIANG
;
Xiangyuan ZHANG
;
Xiangkun JIANG
Author Information
1. 山东省聊城市疾病预防控制中心细菌性传染病预防控制所，聊城 252000
- Keywords:
Yersinia enterocolitica;
Whole genome sequencing;
Virulence gene;
Drug resistance;
Single nucleotide polymorphism typing
- From:
Chinese Journal of Epidemiology
2023;44(2):302-309
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the genome analysis and molecular typing of foodborne Yersinia enterocolitica ( Y.e) strains in Liaocheng City of Shandong Province from 2020 to 2021. Methods:The Y.e strains were isolated from raw meat and meat products. Then we made the strain identification, drug sensitivity test, virulence gene test, pulsed-field gel electrophoresis (PFGE), and whole genome sequencing (WGS). The genome sequencing data were assembled with the microbial genome annotation package. We performed the multilocus sequence typing (MLST) and core genome multilocus sequence typing (cgMLST) and used WGS-based single nucleotide polymorphism typing (wg-SNPs) method to carry out genetic evolution analysis with 14 domestic and Y.e genomes obtained from the NCBI. Results:A total of 21 strains of Y.e were detected from 165 samples, with a detection rate of 12.73%. The 20 strains of Y.e were sequenced successfully. The 20 strains of Y.e carries a variety of drug resistance genes and virulence genes, showing multiple drug resistance. The virulence gene PCR test showed that 21 strains of Y.e having two virulence genes. Cluster analysis of PFGE, MLST, and cgMLST showed that the genomics of 21 strains was highly diverse. The genetic evolution analysis of wg-SNPs showed that 20 Y.e strains could be divided into two main evolutionary branches. Conclusions:Y.e strains isolated from raw meat in Liaocheng City carry a variety of drug resistance genes and virulence genes, and the molecular typing is highly diverse, which may cause infection risk. The molecular biological monitoring of Y.e in raw meat should be strengthened, and genome sequencing and molecular typing detection be carried out to provide the theoretical basis for foodborne illness caused by Y.e.
