DNA Damage Caused By Pesticide-contaminated Soil
- Author:
K.KRISHNAMURTHI
1
;
DEVI SARAVANA S.
;
CHAKRABARTI T.
Author Information
1. National Environmental Engineering Research Institute (NEERI)
- Keywords:
Carbaryl;
Contaminated soil;
DNA damage;
FADU- Huorimetric analysis of DNA unwinding assay;
Genotoxicity;
α-Napthol and pesticide
- From:
Biomedical and Environmental Sciences
2006;19(6):427-431
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the DNA damaging potential and the genotoxicity of individual compounds in pesticide contaminated soil. Methods In the present study, DNA damaging potential of pesticide-contaminated soil and the genotoxicity of individual compounds present in the soil were assessed using fluorimetric analysis of DNA unwinding assay. Results The contaminated soil sample showed 79% (P＜0.001) of DNA strand break, whereas technical grade of major carbaryl and α-naphthol constituents of the contaminated soil showed 64% (P＜0.01) and 60% (P＜0.02) damage respectively. Conclusion Our results indicate that the toxicity caused by contaminated soil is mainly due to carbaryl and α -napthol, which are the major constituents of the soil sample analyzed by GC-MS.
