Genetic characterization analysis of the whole genome sequence of Coxsackievirus A8 associated with hand, foot and mouth disease in China
10.3760/cma.j.cn112338-20201023-01266
- VernacularTitle:手足口病相关柯萨奇病毒A组8型全基因组序列特征分析
- Author:
Dongyan WANG
1
;
Yang SONG
;
Zhenzhi HAN
;
Jinbo XIAO
;
Huanhuan LU
;
Dongmei YAN
;
Tianjiao JI
;
Qian YANG
;
Shuangli ZHU
;
Wenbo XU
;
Yong ZHANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所/国家脊髓灰质炎实验室/世界卫生组织西太平洋区脊髓灰质炎参比实验室/国家卫生健康委生物安全重点实验室/国家卫生健康委医学病毒与病毒病重点实验室，北京102206
- Keywords:
Hand, foot and mouth disease;
Coxsackievirus A8;
Molecular evolution;
Recombination
- From:
Chinese Journal of Epidemiology
2021;42(8):1487-1492
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the genomic sequence of Coxsackievirus A8 (CV-A8) associated with hand, foot and mouth disease (HFMD) from 2013 to 2018 in China and to analyze the genetic evolution of each coding region of the full-length genome.Methods:The genome sequences of 11 CV-A8 strains isolated from patients with HFMD in different regions of China from 2013 to 2018 were determined. Sequence alignment and genetic evolution analysis were performed by Sequencher 5.0 and MEGA 7.0 software, etc.Results:Sequence alignment showed that the genome length of 11 CV-A8 strains ranged from 7 393 bp to 7 400 bp. There was no base insertion or deletion in the coding region compared with the prototype strain, but there were individual base insertion or deletion in the non-coding region. The nucleotide and amino acid similarities in the VP1 region of 11 CV-A8 strains were 78.3%-98.6% and 92.6%-99.7%, respectively, and the nucleotide and amino acid sequences identities with the CV-A8 prototype strain were 78.3%-98.2% and 92.6%-99.7%, respectively. Based on the phylogenetic analysis of VP1 region sequences, the CV-A8 can be divided into five genotypes: A, B, C, D and E. The 11 CV-A8 strains in this study belonged to genotypes C (1 strain), D (2 strains) and E (8 strains). The nucleotide and amino acid similarities of 11 CV-A8 full-length genomes were 81.3%-98.8% and 95.9%-99.5%, respectively. The phylogenetic tree of the P2 region showed that the eight E genotypes CV-A8 had the closest evolutionary distance with CV-A4, CV-A14, and CV-A16. The phylogenetic tree of the P3 region showed that the eight E genotypes CV-A8 had a close evolutionary distance with CV-A5, CV-A16, CV-A14 and CV-A4.Conclusions:The 11 CV-A8 stains in this study showed significant intra-genotype diversity in capsid region and recombinant diversity in non-capsid region which indicated that CV-A8 quasispecies were still undergoing dynamics variation. CV-A8 may become an important pathogen of HFMD and the monitoring of CV-A8 needs to be further strengthened.
