Performance of limiting-antigen avidity enzyme immunoassay and pooling PCR in detection of recent HIV-1 infection among men who have sex with men in Yunnan province
10.3760/cma.j.cn112338-20200605-00810
- VernacularTitle:限制性抗原亲和力酶联免疫法和集合核酸法检测用于云南省哨点监测男男性行为人群HIV-1新发感染的研究
- Author:
Xiaomei JIN
1
;
Huichao CHEN
;
Pengyan SUN
;
Zhijun ZENG
;
Li YANG
;
Chaojun YANG
;
Lijun SONG
;
Zuyang ZHANG
;
Yanling MA
;
Min CHEN
Author Information
1. 云南省疾病预防控制中心性病艾滋病防制所，昆明 650022
- Keywords:
HIV;
Men who have sex with men;
Limiting-antigen avidity enzyme immunoassay;
Pooling PCR;
Recent infection rates
- From:
Chinese Journal of Epidemiology
2021;42(4):706-710
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To assess and compare the performance of limiting-antigen avidity enzyme immunoassay (LAg-Avidity EIA) and pooling PCR in the surveillance for recent infection rates of HIV-1 in men who have sex with men (MSM).Methods:Blood samples were collected from MSM selected through snowball sampling method in sentinel surveillance in 13 prefectures of Yunnan province from 2016 to 2017. The samples were tested for HIV-1 antibody. The confirmed positive samples were tested by LAg-Avidity EIA. The negative samples were tested by pooling PCR. The recent infection rates of HIV-1 were estimated by the algorithm based on LAg-Avidity EIA and pooling PCR respectively. The two results were compared.Results:During 2016-2017, a total of 5 363 blood samples were collected from MSM, in which 407 samples were HIV-1 positive (including 177 positive tested previously) and 4 956 samples were HIV-1 negative. A total of 211 samples(91.7%) were tested by LAg-Avidity EIA, 69 were confirmed to be recent infections. A total of 4 469 samples were tested by pooling PCR, 8 were confirmed to be acute infections. The recent infection rates of HIV-1 from 2016 to 2017 estimated by LAg-Avidity EIA were 3.36% and 4.84%, and the recent infection rates estimated by pooling PCR were 3.27% and 3.02% respectively. The differences in recent infection rates of HIV-1 estimated by the two algorithms were not significant.Conclusions:The recent infection rates of HIV-1 estimated by LAg-Avidity EIA and pooling PCR in sentinel surveillance in MSM in Yunnan had good consistency from 2016 to 2017. Using the two methods might have a better stability in continuous surveillance for recent infection rates of HIV-1.
