Effects of hnRNP E1 on expression of early genes E2, E6 of HPV16 and biological function in cervical cancer cells
10.3760/cma.j.cn112338-20191009-00723
- VernacularTitle:hnRNP E1对HPV16早期基因E2、E6的调控及其对宫颈癌细胞生物学功能的影响
- Author:
Li SONG
1
;
Ling DING
;
Meijuan FENG
;
Xiaoxue LI
;
Wen GAO
;
Zhuo QI
;
Hong LIU
;
Ming WANG
;
Jintao WANG
Author Information
1. 山西医科大学公共卫生学院流行病学教研室，太原030001
- Keywords:
Cervical cancer;
Heterogeneous nuclear ribonucleoproteins;
Human papillomavirus type
- From:
Chinese Journal of Epidemiology
2021;42(2):321-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of hnRNP E1 on the expression of early genes E2, E6 of HPV16 and the biological function in cervical cancer SiHa cell lines.Methods:The cell experiments in vitro were carried out in cervical cancer cell lines SiHa. The expression levels of E2, E6 mRNA and protein of HPV16 were detected by Real-time PCR and Western blot, respectively, before and after up-regulating hnRNP E1. Meanwhile, the cell proliferation, cycle and apoptosis were evaluated by CCK-8 and flow cytometry. Data analyses were performed using SPSS 22.0 and Graphpad Prism 7.0 software. Results:Compared with the blank and the blank plasmid group, the cells activity and proliferation decreased at 24, 48 and 72 h after up-regulating hnRNP E1 ( P<0.05), while the percentage of cells in G0/G1 phase increased and the percentage in S and G2/M phase and proliferation index decreased ( P<0.05). Moreover, the late apoptotic rate and the total apoptotic rate increased ( P<0.05). The expression levels of E6 mRNA and protein of HPV16 in hnRNP E1 up-regulated group were significantly lower than that in both blank group and blank plasmid group, the differences were significant ( P<0.05), showing the tendency of cells proliferation index decrease and total apoptotic rate increase with decreased HPV16 E6 expression. There were no significant differences in the expression of E2 mRNA of HPV16 among the three groups ( P=0.427), and no E2 protein of HPV16 was detected. Conclusions:hnRNP E1 could inhibit the transcription and translation of E6 oncogene of HPV16 and further inhibit the proliferation and promote apoptosis of cervical cancer cells, suggesting that hnRNP E1 might be a potential target marker to prevent cervical lesions. But no association between hnRNP E1 and HPV16 E2 was found in SiHa cells.
