Genotyping on one case with Chikungunya infection introduced into Tianjin in China from Myanmar
10.3760/cma.j.cn112338-20200131-00062
- VernacularTitle:中国天津市一例缅甸输入基孔肯雅热病例的病毒基因分型
- Author:
Tong XIE
1
;
Likun LYU
;
Zhaolin TAN
;
Li LI
;
Jie LYU
;
Xiaoyan LI
Author Information
1. 天津市疾病预防控制中心 300011
- Keywords:
Chikungunya virus;
Chikungunya fever;
Imported case;
Mosquito-borne infectious diseases;
Phylogenetic analyses
- From:
Chinese Journal of Epidemiology
2020;41(12):2131-2134
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:A clinical case caused by Chikungunya virus (CHIKV) was introduced into Tianjin, China from Myanmar. The current study is aimed to phylogenetically analyzing this imported strain and to reveal the relationship between this virus and other circulating CHIKV strains.Methods:RNA was extracted from serum of the suspected patient presenting with symptoms compatible with CHIKV infections. Real-time reverse transcription PCR (RT-PCR) assay was used for diagnoses of the patient. For phylogenetic analysis, envelope glycoprotein 1 (E1) gene of CHIKV was amplified by two-step RT-PCR and the products were sequenced.Results:The phylogenetic analyses revealed that the imported CHIKV belong to Indian Ocean Lineage (IOL) derived from ECSA genotype and sharing the same cluster with the Aede albopitus-adapted strains that triggered the outbreaks in Pakistan (2016), Italy (2017) and Bangladesh (2017). Conclusion:The imported CHIKV strain has the potential to cause explosive outbreaks in China and this event happened in Tianjin calls for strengthening the monitoring programs on mosquito-borne diseases in China.
