Progress on genotyping of norovirus
10.3760/cma.j.cn112338-20200302-00234
- VernacularTitle:诺如病毒基因分型研究进展
- Author:
Lingyu SHEN
1
;
Bo JIANG
;
Hanqiu YAN
;
Weihong LI
;
Zhiyong GAO
;
Quanyi WANG
Author Information
1. 北京市疾病预防控制中心，北京市预防医学研究中心，传染病地方病控制所 100013
- Keywords:
Norovirus;
Genotyping;
Polymerase region;
Capsid region
- From:
Chinese Journal of Epidemiology
2020;41(11):1927-1932
- CountryChina
- Language:Chinese
-
Abstract:
Norovirus are now recognized as one of the main pathogens causing acute gastroenteritis in both developed and developing countries. However, norovirus are easily mutated and recombined, and have many genotypes. In early studies, norovirus were amplified and identified by amino acid sequence of VP1 region. It was found that norovirus were easily mutated and recombined in or near the overlapping regions of polymerase and capsid. A two regions genotyping method was positively proposed internationally. Depending on the 2 times standard deviation standard method for two regions identification, norovirus polymerase regions can be divided into 10 gene groups and 76 genotypes including 2 tentative gene groups and 16 tentative genotypes. The VP1 region can be divided into 12 gene groups and 53 genotypes including 2 tentative gene groups and 5 tentative genotypes. However, the tentative gene groups and genotypes need to be further identified and reclassified. In this article, characteristics of norovirus sequences, principles of different genotyping methods, methods of sequence amplification, on-line genotyping tools and the latest studies in norovirus genotypes are reviewed and introduced.
