Characteristics of HIV-1 genotype and drug resistance among men who have sex with men in Kunming, 2018
10.3760/cma.j.cn112338-20191107-00792
- VernacularTitle:昆明市2018年男男性行为者HIV-1基因型及耐药特征分析
- Author:
Jie DAI
1
;
Pengyan SUN
;
Huichao CHEN
;
Lijuan DONG
;
Xiaomei JIN
;
Youfang LI
;
Jue WANG
;
Chaojun YANG
;
Min YANG
;
Zhijun ZENG
;
Yanling MA
;
Min CHEN
Author Information
1. 云南省疾病预防控制中心性病艾滋病防制所，昆明 650022
- Keywords:
Men who have sex with men;
HIV;
Genotype;
Drug resistance
- From:
Chinese Journal of Epidemiology
2020;41(8):1335-1340
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the characteristics of HIV-1 genotypes and drug resistance among men who have sex with men in Kunming in 2018.Methods:A total of 193 plasma samples were collected from the newly reported HIV-1 infected MSM in Kunming from January to December 2018. Viral RNA was extracted, and the gag, pol, env gene segments were amplified by nested PCR. HIV-1 genotypes and drug resistance were also analyzed. Subsequently, the evolutionary characteristics of CRF55_01B and CRF68_01B among MSM in Kunming were analyzed by Bayesian Markov Chain Monte Carlo method. Results:Multiple HIV-1 genotypes were identified among these 193 samples, including CRF07_BC (39.4%, 76/193), CRF01_AE (34.2%, 66/193), unique recombinant forms (URFs) (20.2%, 39/193), CRF08_BC (3.1%, 6/193), CRF55_01B (1.6%, 3/193), subtype B (1.0%, 2/193) and CRF68_01B (0.5%, 1/193). Results from the Bayesian evolutionary analysis showed that CRF55_01B started to spread locally after being imported from other provinces, while CRF68_01B was likely to have been brought in from the eastern provinces of China. Prevalence of HIV-1 drug resistant strains was 2.6%(5/190) before antiviral treatment, with mutation rates resistant to non-nucleoside reverse transcriptase inhibitors being the highest (2.1%, 4/190) among MSM in Kunming, 2018.Conclusion:The diversity of HIV-1 was increasing among MSM in Kunming. Although the resistance rate on pretreatment drug was relatively low, the emergence of multiple resistant strains to first-line antiviral drugs posed a challenge to antiretroviral therapy, in Kunming.
