Relationship between mutations of HBV basal core promoter region in HBsAg-positive mothers and intrauterine transmission
10.3760/cma.j.cn112338-20200224-00163
- VernacularTitle:HBsAg阳性母亲HBV核心启动子突变与宫内传播的关系
- Author:
Jiaxin WU
1
;
Zhiqing YANG
;
Ruijun ZHANG
;
Yandi LI
;
Tianjing ZHAO
;
Linzhu YI
;
Yongliang FENG
;
Shuying FENG
;
Bo WANG
;
Suping WANG
Author Information
1. 山西医科大学流行病学教研室，太原 030001
- Keywords:
Intrauterine transmission;
Basal core promoter;
HBsAg;
HBeAg;
A1762T/G1764A
- From:
Chinese Journal of Epidemiology
2020;41(6):902-907
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the relationship between maternal mutations in basal core promoter region of hepatitis B virus (HBV) genotype C and intrauterine transmission.Methods:We collected information on general demographic characteristics and process of delivery among 399 pairs of consecutive HBsAg-positive mothers and their neonates, from the Third People’s Hospital of Taiyuan in Shanxi province, China. Fluorescence quantitative polymerase chain reaction (FQ-PCR) and Electro-chemiluminescence immuno-assay (ECLIA) kits were used to detect both maternal and neonatal HBV DNA and serological markers in the peripheral blood. From 113 mothers with HBV DNA load ≥10 6 IU/ml, we selected 22 mothers whose neonates were with intrauterine transmission and randomly selected the same number of mothers whose neonates were without intrauterine transmission, as controls. The whole-length HBV DNA were extracted, amplified, cloned, sequenced and genotyped. Finally, a total of 39 mothers with genotype C of HBV were selected for mutation analysis. Results:Thirty-nine cases of genotype C (88.63 %) were finally included in the study, with 19 cases in the intrauterine transmission group and 20 cases as controls. Rates of A1762T/G1764A double mutations were significantly different between the intrauterine transmission group and the control group (7.53 % vs. 27.72 %, P<0.001). Results from the multivariate analysis showed that the A1762T/G1764A double mutations had reduced the risk of intrauterine transmission (a OR=0.065, 95 %CI: 0.006-0.746, P=0.028). Maternal A1762T/G1764A double mutations appeared to be possibly associated with neonatal HBeAg ( P=0.050). Conclusion:A1762T/G1764A double mutations of HBV DNA from the genotype C of those HBsAg-positive mothers could reduced the risk of HBV intrauterine transmission during pregnancy.
