Analysis on drug sensitivity spectrum of 167 multidrug-resistant Mycobacterium tuberculosis in China
10.3760/cma.j.cn112338-20191121-00823
- VernacularTitle:167株耐多药结核分枝杆菌药物敏感谱分析
- Author:
Rong CHEN
1
;
Machao LI
;
Lili ZHAO
;
Xiuqin ZHAO
;
Haican LIU
;
Zhiguang LIU
;
Yao LU
;
Yunli DENG
;
Zixin CHEN
;
Kanglin WAN
;
Xiuqin YUAN
Author Information
1. 南华大学公共卫生学院，衡阳 421001；中国疾病预防控制中心传染病预防控制所 传染病预防控制国家重点实验室 感染性疾病诊治协同创新中心，北京 102206
- Keywords:
Multidrug-resistant tuberculosis;
Drug sensitivity test;
Cross resistance;
Genotype
- From:
Chinese Journal of Epidemiology
2020;41(5):764-769
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the drugs-sensitivity spectrum of multidrug-resistant tuberculosis (MDR-TB) in China and provide a scientific evidence for the drug selection in clinical therapy and the control of MDR-TB.Methods:A total of 167 strains of MDR-TB were included in this study. Every strain was genotyped by lysX gene sequencing and their sensitivity to 13 different anti-TB drugs was tested by using MicroDST TM and BACTEC TM MGIT 960 TM liquid-culturing method. The association between drug resistance and genotypes as well as cross drug resistance was also analyzed. The results were analyzed by means of the comparison of enumeration data between two groups with χ2 test. Results:The overall resistance rate of 167 MDR-TB strains to 11 anti-TB drugs, except isoniazide and rifampicin, was 95.81%, the rates of pre-extensive drug-resistance (pre-XDR) and extensive drug-resistance were 31.14%(52/167) and 6.59% (11/167), respectively. The streptomycin resistance rate of Beijing genotypes was significantly higher than that of the non-Beijing genotypes ( χ2=30.682, P<0.05), while the pre-XDR proportion in Beijing genotypes was lower than that in non-Beijing genotypes ( χ2=5.332, P<0.05). The resistance rates of Ofloxacin and Pyrazinamide in the modern Beijing genotype were significantly higher than those in classical ones ( χ2=4.105 and χ2=3.912, P<0.05). In addition, the cross-resistance rate to rifampicin and rifabutin was 86.23%. A significant difference in drug-resistance rate to rifabutin was seen among groups with different levels of rifampicin resistance ( χ2=45.912, P<0.05). There was positive correlation not only between ofloxac resistance and moxifloxac resistance, but also between amikacin resistance and kanamycin resistance, with the coefficient of 0.87 and 0.91, respectively. Conclusions:In this study, we observed that there were high incidences of the resistance to 11 anti-TB drugs in 167 clinical MDR-TB strains and the cross resistance phenomena between drugs of the same type were quite serious. The majority of MDR-TB strains belonged to Beijing genotype, which was highly associated with streptomycin resistance.
