Emerging of Japanese encephalitis virus and Getah virus from specimen of mosquitoes in Inner Mongolia Autonomous Region
10.3760/cma.j.cn112338-20190425-00284
- VernacularTitle:内蒙古自治区采集的蚊虫标本新发现乙脑病毒和盖塔病毒
- Author:
Rui CHENG
1
;
Xiaowei NAN
;
Na FAN
;
Shihong FU
;
Xiaoyan SI
;
Lin ZHANG
;
Ying HE
;
Wenwen LEI
;
Fan LI
;
Huanyu WANG
;
Xiaoqing LU
;
Guodong LIANG
Author Information
1. 青岛大学公共卫生学院 266071；中国疾病预防控制中心病毒病预防控制所病毒性脑炎室 传染病预防控制国家重点实验室，北京 102206
- Keywords:
Japanese encephalitis;
Getah virus;
Arbovirus
- From:
Chinese Journal of Epidemiology
2020;41(4):571-579
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the types and distribution of blood-sucking insects and arboviruses in Inner Mongolia autonomous region, and provide basic data for the prevention of arbovirus transmitted disease.Methods:Blood-sucking insects were collected by lamp trapping method in nature. Mosquito samples were classified according to morphologic characteristics and then stored at liquid nitrogen. Viruses were isolated in cell culture and characterized, using molecular biological methods.Results:A total of 24 240 mosquitoes and 17 110 aphids were collected from 2 sites of 5 counties (Flags) in Inner Mongolia in 2014 and during 2017-2018. Among them, Japanese encephalitis virus gene was detected in Culex pipiens pallens, and 4 virus strains isolates which could be stably passaged. The isolates were identified as Getah virus and densonucleosis virus by molecular biology identification. Phylogenetic analysis on the E2 gene of the Getah virus (NMDK1813-1) showed that it belonged to the same evolutionary branch of the Gansu isolates (GS10-2) and having six common amino acid variation sites. Conclusions:The emergence of Japanese encephalitis virus and Getah virus from specimen of mosquitoes in Inner Mongolia indicated the new challenges on the prevention and control of arbovirus and related diseases. The results pf this study provided basic data for the prevention and control stretagies of arbovirus transmitted diseases in Inner Mongolia.
