Isolation and identification of Arbovirus in Hainan province, 2017-2018
10.3760/cma.j.issn.0254-6450.2020.02.018
- VernacularTitle:海南省2017-2018年虫媒病毒的分离与鉴定
- Author:
Na FAN
1
,
2
;
Dingwei SUN
;
Rui CHENG
;
Shihong FU
;
Linhai ZENG
;
Qun WU
;
Shan'gan LI
;
Ying HE
;
Wenwen LEI
;
Fan LI
;
Huanyu WANG
;
Xiaoqing LU
;
Guodong LIANG
Author Information
1. 青岛大学医学院公共卫生学院职业卫生与环境卫生教研室 266071
2. 中国疾病预防控制中心病毒病预防控制所病毒性脑炎室
- Keywords:
Arbovirus;
Japanese encephalitis virus;
Getah virus;
Akabane virus
- From:
Chinese Journal of Epidemiology
2020;41(2):236-243
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the types and distribution of Arboviruses in Hainan province.Methods Blood-sucking insects were collected in Hainan province from 2017 to 2018.After laboratory treatment,BHK-21 cells and C6/36 cells were inoculated with grinding supernatant of all blood-sucking insects to isolate all of involving virus.Arbovirus genes in blood-sucking insects were detected in parallel by RT-PCR method.Results A total of 15 062 mosquitoes were classified into four genera (Culex,Armigeres,Aedes,Anopheles) and 11 360 midges were collected.Culex tritaeniorhynchus was in the majority and accounted for 92.88％ (13 990/15 062) of all the mosquitoes collected.Four strains of virus isolates were notified by tissue culture method.Three strains of viruses belonged to Japanese encephalitis virus (JEV),with the other one as Getah virus (GETV).Five pools of JEV gene amplification were positive,from Culex tritaeniorhynchus.Results from the phylogenetic analysis showed that they belonged to genotype JEV-Ⅰ.The minimum infection rate of JEV was 0.57‰ (8/13 990).A total of 5 pools of Akabane virus (AKV) gene amplification were positive.The minimum infection rate of AKV was 0.44‰ (5/11 360).Based on the S gene and M gene sequences of the virus,data from the phylogenetic analysis showed that the five AKV strains carried by midges in Hainan province were in a separate evolutionary branch and with formed unique geographical distribution.Conclusions JEV and GETV had been isolated again from the mosquito specimens in this survey,since the 1980s.AKV was detected from the midge specimens in Hainan province.These results showed the needs of strengthening the programs on detection and monitor of JEV,GETV and AKV that were related to animal and human diseases in order to reduce the risks of related diseases in this area.
