Preliminary study on the expression and distribution of S100A8 and S100A9 in healthy and experimental periodontitis tissues
10.3760/cma.j.cn112144-20191219-00456
- VernacularTitle:S100A8、S100A9蛋白在健康和实验性牙周炎组织中的表达分布初探
- Author:
Hongyu GAO
1
;
Baoxin HUANG
;
Jianxia HOU
;
Huanxin MENG
Author Information
1. 北京大学口腔医学院·口腔医院牙周科 国家口腔疾病临床医学研究中心 口腔数字化医疗技术和材料国家工程实验室 口腔数字医学北京市重点实验室 100081（现在天津医科大学口腔医院牙周科 300070）
- Keywords:
Periodontitis;
S100A8;
S100A9;
Gingival fibroblast;
Periodontal ligament cell
- From:
Chinese Journal of Stomatology
2020;55(6):402-407
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the systemic expression profile of S100A8 and S100A9 in healthy and inflamed periodontal tissues.Methods:Experimental periodontitis models were established by ligations around the mandibular second molars of six beagle dogs for 12 weeks (ligation group). The mandibular second molars on the opposite side were kept clean (healthy control group). The expressions of S100A8 and S100A9 in healthy and inflamed periodontal tissues of six beagle dogs were examined by immunohistochemistry. The expressions of S100A8 and S100A9 in primary human gingival fibroblasts (hGF) from 3 subjects and human periodontal ligament cells (hPDLC) from 3 other subjects were detected by immunocytochemistry.Results:After the ligation for 12 weeks, the mean probing depth of ligation group [(3.86±0.14) mm] was significantly higher than that of healthy control group [(2.11±0.28) mm] ( P<0.01). Results of immunohistochemistry analysis indicated that S100A8 and S100A9 could be expressed in gingival epithelial cells and might infiltrated neutrophils in the healthy periodontium. Except for the gingival epithelial cells and neutrophils, both proteins were induced and expressed in gingival fibroblasts, periodontal ligament cells, microvascular endothelial cells and bone marrow fibroblasts under inflammatory conditions. The distribution of S100A8 and S100A9 differed in the healthy oral gingival epithelium (OGE), which becomes consistent in inflamed OGE. Additionally, the expressions of S100A8 and S100A9 were confirmed in primary hGF and hPDLC. Conclusions:Periodontal inflammation might enlarge the expression scope of S100A8 and S100A9 and enrich multiple cells with expressions of S100A8 and S100A9.
