Effect of magnesium and selenium on the expression of matrix metalloproteinases-20 and kallikrein 4 in fluorosis mice
10.3760/cma.j.issn.1002-0098.2016.09.008
- VernacularTitle:镁硒对氟中毒小鼠基质金属蛋白酶20及激肽释放酶4表达的影响
- Author:
Feng WANG
1
;
Tiezhou HOU
;
Jiejie LI
;
Zixia LI
;
Chengfang TANG
Author Information
1. 西安医学院口腔医学系口腔内科学教研室
- Keywords:
Fluorosis,dental;
Matrix metalloproteinases 20;
Kallikreins;
Magnesium;
Selenium
- From:
Chinese Journal of Stomatology
2016;51(9):546-551
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of magnesium/selenium on the expression of matrix metalloproteinases-20(MMP-20) and kallikrein 4(KLK4) during fluorosis in mice and to explore the formation mechanism of dental fluorosis.Methods Eighty SPF male ICR mice were randomly divided into 8 groups according to body weight:control group,magnesium group,selenium group,magnesium-selenium group,fluoride group,magnesium-fluorine group,selenium-fluorine group and magnesium-selenium-fluorine group.Mice in control,magnesium,selenium and magnesium-selenium groups were fed double steamed water,and mice in the other four groups were feddouble steamed water with 50 mg/L F.Mice in control and fluoride groups were fed conventionally.Mice in magnesium and magnesium-fluorine groups were fed conventionally by adding MgSO4·7H2O 162.5 mg/kg.Mice in selenium and selenium-fluorine groups were fed conventionally by adding Na2SeO3·5H2O 2 mg/kg.Mice in magnesium-selenium and magnesium-selenium-fluorine groups were fed conventionally by adding MgSO4· 7 H2O 162.5 mg/kg + Na2SeO3·5H2O 2 mg/kg.Incisor specimens were obtained after the mice were put into death when they were 42 days.The expressions of MMP-20 and KLK4 were observed by using immunohisto-chemicalstain.Results The meangray value of MMP-20 of fluoride group(133.1±10.3) was significantly higher than that of control group(116.8±10.0),magnesium group (113.6 ± 9.6),magnesium-selenium group(108.2 ± 15.2),magnesium-fluorine group(Ill.1 ± 8.1) and magnesium-selenium-fluorine group(108.2 ± 11.0),respectively(F=3.864,P＜0.05).The mean gray value of MMP-20 of magnesium-selenium-fluorine group(108.2 ± 11.0) was significantly lower than that of selenium group(125.4 ± 7.9),fluoride group (133.1 ± 10.3) and selenium-fluorine group(126.2 ± 2.8),respectively(F=3.864,P＜0.05).The mean gray value of KLK4 of magnesium-selenium group(117.2± 11.7) was significantly lower than others(137.3±7.9 of control group,144.2±7.7 of magnesium group,138.9±13.3 of selenium group,149.7± 12.4 of fluoride group,148.9±7.5 of magnesium-fluorine group,140.6± 17.0 of selenium-fluorine group and 140.7 ± 7.3 of magnesium-selenium-fluorine group,F=3.668,P＜0.05).In factorial analysis of fluorosis mice,magnesium had effect on the expression of MMP-20(F=42.613,P＜0.05),selenium had effect on the expression of KLK4(F=6.649,P＜0.05).Conclusions The excessive fluoride could inhibit the expressions of MMP-20.The excessive fluoride hadno significant influence on the expression of KLK4.Magnesium and selenium had antagonistic effect on the dental fluorosis.
