Effects of exogenous enzymes on the degradation of adhesive-dentin interfaces
10.3760/cma.j.issn.1002-0098.2016.04.009
- VernacularTitle:两种外源性酶对牙本质粘接界面的老化作用初探
- Author:
Donglai DENG
1
;
Hongye YANG
;
Jingmei GUO
;
Cui HUANG
;
Jing GAN
;
Fangfang SONG
Author Information
1. 430022,武汉大学口腔医学院中心门诊
- Keywords:
Collagenases;
Sterol esterase;
Dental bonding;
Bonding durability
- From:
Chinese Journal of Stomatology
2016;51(4):230-234
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of exogenous enzymes on the degradation of adhesive-dentin interface.Methods Forty molars were sectioned to expose the middle-coronal dentin surface and randomly divided into two adhesive systems:an etch-and-rinse adhesive Adper Single Bond 2 and a self-etching adhesive G-Bond.After composite building up,the specimens were then randomly assigned to four groups(n=5 for each group) as follows:group 1,24 h of water storage(the control group);group 2,six months of water storage;group 3,twelve weeks storage in artificial saliva containing clostridium histolyticum collagenase;group 4,twelve weeks storage in artificial saliva containing cholesterolesterase.The microtensile bond strengths(MTBS) were then tested.The failure modes and nanoleakage were analyzed.Results After aging treatments,the three aging groups showed significantly lower MTBS compared with the control group in both adhesive systems(P＜0.05).For etch-and-rinse adhesive Adper Single Bond 2,the MTBS of group 3([19.6±3.5] MPa) was lower than that of group 2([23.4±4.2] MPa) and group 4([24.2±4.2] MPa) (P＜0.05).For self-etching adhesive G-Bond,there was no difference on MTBS among different aging groups (P＞0.05).SEM observation showed that,compared with the control group,water storage(group 2) and the exogenous enzymes(group 3 and 4) increased the nanoleakage expression(silver deposition) of both adhesive systems.Adhesive failure was the predominant fracture modes in all groups.Conclusions Storage in artificial saliva containing clostridium histolyticum collagenase or cholesterol esterase could be used to accelerate the degradation process of adhesive-dentine interface.
