Porphyromonas gingivalis FimA genotype distribution among periodontitis patients with type 2 diabetes
10.3760/cma.j.issn.1002-0098.2016.01.006
- VernacularTitle:伴糖尿病牙周炎患者牙龈卟啉单胞菌FimA基因型的检测
- Author:
Bingtao YANG
1
;
Jingling XU
;
Lu HE
;
Huanxin MENG
;
Li XU
Author Information
1. 100081,北京大学口腔医学院·口腔医院牙周科
- Keywords:
Periodontitis;
Diabetes mellitus,type 2;
Porphyromonas gingivalis;
Genotype
- From:
Chinese Journal of Stomatology
2016;51(1):20-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution differences of Porphyromonas gingivalis(Pg) FimA genotypes between periodontitis patients with and without type 2 diabetes for a better understanding of the relationship between diabetes and periodontitis.Methods Questionnaires and detailed periodontal examinations(6 sites per tooth) were conducted in 80 subjects with moderate-severe chronic periodontitis in the Department of Periodontology, Peking University School and Hospital of Stomatology.There were 40 type 2 diabetic patients and 40 systemicly healthy patients enrolled respectively.The periodontal parameters including plaque index(PLI), probing depth(PD), bleeding index(BI), attachment loss(AL) by 6 sites per tooth and numbers of missing teeth were also recorded.Pooled subgingival plaque samples using pocket method with Whatman No3 filterpaper were collectedat each of the 6 sites from one incisor and one molar.Pg and its FimA genotype distributions were investigated using DNA extrctedfrom plaque samples by PCR.Results Diabetic patients had a significantly higher score of PLI[2.35(0.58) vs 1.64(0.76), P＜0.05] , while rest of periodontal indexes observed(PD, BI and AL) did not differ significantly between diabetic patients and systemicly healthy controls(P＞0.05).The detection rate of Pg did not show statistically significant difference between the two groups(50％ vs 60％, P＞0.05).However, the proportion of FimA Ⅱ was significantly higher in diabetic group than systemicly healthy group(80％ vs 42％, P＜0.05).Conclusions Type 2 diabetic patients were prone to be infected by highly virulent strains of Pg: FimA Ⅱ Pg.
