Effect of calcium and phosphate ion concentration from calcium phosphate cement leaching liquor on osteoblast : observation by calvaria organ culture method
10.3760/cma.j.issn.1002-0098.2013.s1.025
- VernacularTitle:利用器官培养方法观察磷酸钙骨水泥浸提液对成骨细胞的影响
- Author:
Chong-Ying ZHAO
1
;
Ling-Fei WANG
;
Yu-Ying MAI
;
Hui-Huang WU
;
Hong-Bing LIAO
Author Information
1. 广西医科大学口腔医学院口腔修复科
- Keywords:
Calcium phosphates;
Organ culture techniques;
Skull;
Osteoblasts
- From:
Chinese Journal of Stomatology
2013;48(z1):111-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of calium and phosphate ion concentration from calcium phosphate cement leaching liquor on osteoblast by using newborn rats calvarial organ culture model in vitro,and to provide an objective model for the research on calcium phosphate cement degradation and osteoconduction.Methods Neonatal rat calvarial were treated with three types of media separately:BGJb media(blank control group),BGJb media + leaching liquoron of leaching 6 hours(experimental group 1),BGJb media + leaching liquoron of leaching 14 days(experimental group 2).Proliferation of osteoblast was determined by methyl thiazolyl tetrazolium (MTT).Differentiation of osteoblast was evaluated by the following assays:alkaline phosphatase (ALP) assay,and the real-time reverse transcription PCR.Results In experimental group 1,the proliferation(the A value were 0.563 ± 0.047,0.932 ± 0.065,1.325 ± 0.139,1.395 ±0.108 in 3,5,7,9 days respectively),the ALP activity[(5.111 ±0.440),(8.380 ±0.505),(10.124 ± 0.625) U/g prot in 3,7,10 days respectively) and osteocalcin expression of osteoblast (the 2-ΔΔct value were 4.41 ±0.52,21.24 ±2.17,31.84 ±3.51 in 3,7,10 days respectively) inside cultured calvarial were enhanced over time,while in experimental group 2,the activities of osteoblast revealed an inhibiting profile in 5,7,9 days,and the ALP activity and osteocalcin expression of osteoblast decreased compared to the blank control group in 7,10 days.Conclusions Calvarial organ culture method can be used as the research model for understanding the degradation of calcium phosphate bone cement.
