Rapid quantification of periodontal bacteria using an impedic immunosensor
10.3760/cma.j.issn.1002-0098.2013.s1.019
- VernacularTitle:快速量化牙周细菌的阻抗免疫传感器研究
- Author:
Zhen-Hua PEI
1
;
Zhong-Ying NIU
;
Sheng-Gen SHI
;
Chu-Hua TANG
Author Information
1. 解放军306医院全军口腔疾病诊治中心
- Keywords:
Electric impedance;
Periodontal bacteria;
Immunosensor
- From:
Chinese Journal of Stomatology
2013;48(z1):87-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an electrochemical impedance immunosensor based on polypyrrole modified microelectrodes for periodontal bacteria rapid quantification.Methods Mcirofluidic chip with embedded IDAM was prepared by conventional photolithography process.Then polypyrrole structure was deposited on microelectrodes through electropolymerization method.Polypyrrole was biofuncationalized with mouse anti-Porphyromonas gingivalis gingipain K IgG antibody using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide-hydrochloride (EDC) chemistry.The linear relationship between the impedance and bacterial concentration,the impedance characteristic and the feasibility of the developed impedic immunosensor in quantifing Porphyromonas gingivalis was investigated respectively.Results The polypyrrole membrane structure was observed on work electrodes,and the lowest detection limit of the immunosensor was 107 cells/L in pure culture.In the concentration range of 107-1012 cells/L,a linear relationship was found between the normalized impedance change and the logarithmic value of the cell concentration.The total detection time from sampling to measurement was less than 1 h.Conclusions The immunosensor developed in the present study offered some insight into chair-side quantifing periodontal bacteria.
