Effect of Ginkgo biloba extract on the proliferation and expression of cytokine in human periodontal ligament cells treated with lipopolysaccharide
10.3760/cma.j.issn.1002-0098.2013.s1.017
- VernacularTitle:银杏叶提取物对脂多糖作用下人牙周膜细胞增殖及分泌细胞因子的影响
- Author:
Wei-Kun HUANG
1
;
Xiao-Yan GUAN
;
Jian-Guo LIU
;
Ning-Jing KONG
;
Qin FAN
;
Di ZHANG
Author Information
1. 贵州省人民医院口腔科
- Keywords:
Lipopolysaccharides;
Cytokines;
Ginkgo biloba extract;
Periodontal ligament cells
- From:
Chinese Journal of Stomatology
2013;48(z1):77-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Ginkgo biloba extract(GBE) on the proliferation and expression of tumor necrosis factor-a(TNF-α),interleukin(IL)-6,IL-1 β in human periodontal ligament cells (PDLC) treated with lipopolysaccharide (LPS).Methods Human PDLC were cultured with modified tissue culture method.Methyl thiazolyl tetrazolium (MTT) colorimetric assay was used to assess the cell proliferation.Enzyme-linked immunosorbent assay (ELISA) was applied to detect the level of TNF-α,IL-6,IL-1β in the culture supernatant.One-way ANOVA and LSD-t were used to analyze the data.Results The absorbance in LPS +0.1 g/L GBE group was 0.30 ±0.03,0.33 ±0.02,0.34 ±0.02,0.35 ±0.02 at 12,24,48 and 72 h,respectively,higher than that in LPS group (0.20 ± 0.03,0.20 ± 0.02,0.22 ± 0.04,0.24 ± 0.02) (P ＜ 0.05).IL-6,IL-1β and TNF-α in PDLC were lower than that in LPS group (P ＜ 0.05).The absorbance in LPS +0.01 g/L GBE group was 0.27 ±0.05,0.31 ±0.03,0.33 ±0.03,0.32 ±0.01,higher than that in LPS group (P ＜ 0.05).IL-6,IL-lβ and TNF-α in PDLC were lower than LPS group (P ＜ 0.05).Conclusions GBE has the protective effects on human PDLC via inhibiting LPS.
