Analysis of the influence of fluoride foam on oral plaque microbiota by PCR-based denaturing gradient gel electrophoresis
10.3760/cma.j.issn.1002-0098.2013.s1.006
- VernacularTitle:变性梯度凝胶电泳分析氟化泡沫对牙菌斑微生物的影响
- Author:
Jin-Long WENG
1
;
He-Chuan ZHAO
;
Feng SUN
;
Xiao-Chi CHEN
;
Tao XU
Author Information
1. 100034,北京大学口腔医学院·口腔医院第一门诊部
- Keywords:
Fluorides,topical;
Dental caries;
Denaturing gradient gel electrophoresis;
Flora diversity
- From:
Chinese Journal of Stomatology
2013;48(z1):23-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of 1.23％ fluoride foam on microflora in dental plaque of children.Methods Ten children whose average age was 3.7 years from a kindergarten in Beijing city were selected in this study.Oral examinations were conducted and decayed,missing,filled surface (dmfs) value were recorded at baseline.Subjects were applied with 1.23％ fluoride foam.The buccal plaque samples from bilateral maxillary primary molars (V IV | IV V) were collected before and after the fluoride application at baseline,1st,2nd,3rd,4th,7th,10th,15th,21th,30th day.The total microbial genomic DNA of the plaque was isolated.PCR was performed with a set of universal bacterial 16S rDNA primers.The PCR amplified 16S rDNA fragments were separated by denaturing gradient gel electrophoresis (DGGE).The relationship between microflora was assessed by comparing the PCR-DGGE fingerprinting profiles.Results The mean species richness of the bacterial population was 26.9 ± 2.9 at baseline,and fell to 20.1 ± 3.8 after three days of using fluoride foam.The difference was statistically significant (P ＜ 0.05).The overall diversity of plaque samples as measured by the Shannon-Weiner index was 3.18 ±0.31 at baseline and 2.92 ± 0.28 at 3rd day.The difference was statistically significant (P =0.04 by the Mann-Whitney U test).The microbial diversity was changed after the fluoride application by Dice coefficient and clustering analysis.After one month,the microbial diversity was similar to the baseline.Conclusions The results suggest that 1.23％ fluoride foam has obviously inhibiting effects on microbial diversity in dental plaque of children during a period of time.The microbial diversity and complexity of the microbial biota in dental plaque were changed after fluoride application and was able to return to original state.
