Expression of autophagy in oral squamous cell carcinoma and its effect on Tca83 cell line
10.3760/cma.j.issn.1002-0098.2012.s1.053
- VernacularTitle:自噬在Tca83细胞中的作用及自噬相关基因的表达
- Author:
Ze-Bing ZHANG
1
;
Hai-Ying QUAN
;
Tong-Fei ZHANG
;
Yu BAI
;
Xuan-Yi ZHAO
Author Information
1. 吉林大学口腔医学院病理科
- Keywords:
Mouth neoplasms;
Autophagy;
Cisplatin;
Apoptosis
- From:
Chinese Journal of Stomatology
2012;47(z1):233-238
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of autophagy in oral squamous cell carcinoma and its effect on oral squamous cell carcinoma Tca83 cell line.Methods Expression of Beclin-1 and rat microtubule-associated protein light chain-3 (MAP1LC3) were detected in oral squamous cell carcinoma and pericancerous tissues by immunohistochemical (IHC).After treated with cisplatin at different time,Tca83 cells survival rate was examined by methyl thiazolyl tetrazolium(MTT) colorimetry.Autophagosome formation was detected by laser scanning confocal microscope.Apoptosis rate was checked by flow cytometry.Results The expressin of Beclin-1 was found in 40％ (19/47) of the oral squamous cell carcinoma and pericancerous tissues(12/16),and the expression of MAP1LC3 in oral squamous cell carcinoma and peficancerous tissues were 30％ (14/47) and 10/16 respectively.The expression of Beclin-1 and MAP1LC3 in oral squamous cell carcinoma was significantly lower than in peficancerous tissues (P ＜ 0.05).MTT results showed that compared with control group,the survival rate of the Tca83 ceils treated by cisplatin reduced with time (P ＜ 0.05).Immunofluorescence results showed that the average fluorescence intensity of control group (213 ± 20) was significantly lower than those of the other five groups (2 h group:233 ± 16,6 h group:315 ± 27,12 h group:298 ±46,24 h group:259 ± 36,48 h group:225 ± 27) (P ＜ 0.05).Flow cytometry showed that compared with control group,the apoptosis rate increased with time (control group:0.68％,2 h group:2.25％,6 h group:3.08％,12 h group:5.23％,24 h group:7.56％,48 h group:9.46％) (P ＜ 0.05).Conclusions Autophagy may be related to the occurrence and development of oral squamous cell carcinoma.
