Effect of direct tooth-colored restorative materials on early adhesion of Streptococcus mutans
10.3760/cma.j.issn.1002-0098.2012.s1.042
- VernacularTitle:变形链球菌在三种直接修复用牙色材料抛光表面的早期黏附观察
- Author:
Chong-Yang YUAN
1
;
Xiao-Yan WANG
;
Xue-Jun GAO
Author Information
1. 100081,北京大学口腔医学院·口腔医院牙体牙髓科
- Keywords:
Dental materials;
Bacterial adhesion;
Microscopy,confocal
- From:
Chinese Journal of Stomatology
2012;47(z1):181-185
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate early adhesion of Streptococcus mutans (Sm) (UA159) on the surface of direct tooth-colored restorative materials.Methods Three direct tooth-colored restorative materials,composite resin (CR,Filtek Z350,3M ESPE,America),pre-reacted glass ionomer (Giomer,Beautifil Ⅱ,Shofu,Japan) and resin-modified glass ionomer (RMGIC,Fuji Ⅱ LC,GC,Japan),were placed in fabricated models(8 mm × 8 mm ×2 mm),covered with mylar film and light-cured.The samples (n =5) were polished with disks in series (grit 60 μm、grit 30 μm、grit 20 μm、grit 7μm) (Supersnap,Shofu,Japan),then coated with artificial saliva for 2 h.Adhesion of Sm(UA159) on the surface of samples was investigated using confocal laser scanning microscopy(CLSM) in conjunction with fluorescence staining technique after 2 h.The area covered by dead and vital adhesive bacteria(Ad,Av) and dead/vital bacteria ratio(Ad/Av) of different materials were measured with Image-Pro Plus 5.1 and compared using SPSS (K-Independent-Samples Tests/Kruskal-Wallis H).Results Under CLSM,adhesive bacteria,especially vital ones,were observed on the surface of all the three materials.Compared with RMGIC,CR and Giomer showed less dead and vital bacterial adhesion (P ＜ 0.05):Ad [M (Q)]:Filtek Z350 [0.007％(0.037％)],Beautifil Ⅱ [0.011％ (0.028％)] ＜ FujiⅡ LC[0.082％(0.082％)].Av[M(Q)]:Filtek Z350 [0.103％ (0.162％)],Beautifil Ⅱ [0.067％ (0.181％)] ＜ Fuji Ⅱ LC [0.690％(0.482％)].However,Ad/Av[M(Q)] of Beautifil Ⅱ [0.250(0.188)] was lower than the other two,Filtek Z350[0.069(0.125)] and FujiⅡ LC[0.114(0.051)] (P＜0.05).Conclusions Early adhesion of Sm on the surface of direct tooth-colored restorative materials were material type dependent.
