Comparation of 1α, 25-dihydroxyvitamin D3-stimulated interleukin 1 receptor type Ⅰ expression in young and aged human periodontal ligament cells
10.3760/cma.j.issn.1002-0098.2012.s1.034
- VernacularTitle:1α,25-羟基维生素D3对人牙周膜细胞Ⅰ型白介素1受体表达的影响
- Author:
Xiao-Tong LI
1
;
Yan-Qi YANG
;
Ning DU
;
Min-Kui FU
Author Information
1. 100081,北京大学口腔医学院·口腔医院正畸科
- Keywords:
Calcitriol;
Age factors;
Periodontal ligament cell;
Interleukin 1 receptor
- From:
Chinese Journal of Stomatology
2012;47(z1):149-152
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the 1 α,25-dihydroxyvitamin D3 (VD3)-stimulated interleukin 1 receptor,type Ⅰ (IL-1R type Ⅰ) expression in young and aged human periodontal ligament cells.Methods Osteoclast formation was examined in coculture of mouse bone marrow cells and calvariae ostecblasts in present of VD3.VD3-stimulated IL-1 R type Ⅰ expression was examined by reverse transcription polymerase chain reaction(RT-PCR) method in mouse bone marrow cells,calvariae ostecblasts,3rd-5th passages and 17th-20th passages human periodontal ligament cells.Results VD3 enhanced osteoclast formation powerfully in cocuhure of mouse bone marrow cells and calvariae ostecblasts.Osteoblasts were the response cells for VD3 stimulati in IL-1R type Ⅰ expression in coculture sytstem.VD3 stimulated IL-1R type Ⅰexpression in young periodontal ligament cells.The ratio of IL-1 R type Ⅰ and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was 41％,but not aged human periodontal ligament cells.Conclusions The non-response of aged periodontal ligament cells for VD3-stimulated IL-1 R type Ⅰ expression could be related to the delay in the onset of alveolar bone resorption and tooth movement.
