Effect of focal adhesion kinase on the mRNA expression of transcription factors c-fos/c-jun in cultured human periodontal ligament fibroblasts loaded with tensional force
10.3760/cma.j.issn.1002-0098.2012.s1.033
- VernacularTitle:动态张应力下黏着斑激酶对人牙周膜成纤维细胞转录因子c-fos和c-jun mRNA表达的影响
- Author:
Qing-Dang ZHU
1
;
Li LIU
;
Jun HU
Author Information
1. 浙江大学医学院附属口腔医院修复科
- Keywords:
Periodontal ligament;
Fibroblasts;
Stress,mechanical;
Transcription factors;
Focal adhesion kinase
- From:
Chinese Journal of Stomatology
2012;47(z1):145-148
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate the effect of focal adhesion kinase (FAK) on the mRNA expression of transcription factors c-fos/c-jun in cultured human periodontal ligament fibroblasts (hPDLF)loaded with tensional force.Methods The periodontal ligament,which was attached to the mid-third part of the fresh root of young premolars extracted for orthodontic purposes,was removed.The hPDLF was cultured using the method of digesting by Ⅰ-type collagenase combined with tissue adhering.Then hPDLF was isolated and purified by cells passage and PDLF were used in the experiments at passage six.A four-point bending device named " Forcel" was used for force loading.Cells cultured in vitro were loaded with 2000 μstrain tensional force and collected at different time (0,0.5 h,1 h,2 h,or 4 h) after strain loading.Cells were divided into two groups (with or without FAK antibody).Aherations in the mRNA expression of transcription factors c-fos/c-jun due to cyclic tensional force were investigated using real-time polymerase chain reaction (PCR).ANOVA and LSD were used to process the data obtained.Results After strain loading the c-fos/c-jun mRNA expression was upregulated,with a peak at 0.5 h,and then downregulated after 0.5 h.The levels of gene expression in cells treated with the FAK antibody were greater than those in cells untreated with the FAK antibody (P ＜0.05).Conclusions These results provided evidence that transcription fac tors c-fos/c-jun probably played a critical part in the response of periodontal tissue (periodontal ligament fibroblasts) to mechanical stimulation and that FAK probably played an important role in the mechanical signaling pathway of hPDLF.
