Effect of berberine on the cell cycle of Candida albicans

VernacularTitle:小檗碱对白色念珠菌细胞周期的影响
Author: Xue-Min SHEN ¹ ; Ting-Jun XUE ; Lin-Jun SHI ; Wei LIU ; Zeng-Tong ZHOU
Author Information

1. 210011,上海交通大学医学院附属第九人民医院口腔医学院口腔黏膜科上海市口腔医学研究所上海市口腔医学重点实验室
Keywords: Candida albicans; Cell cycle; Berberine; Flow cytometry
From: Chinese Journal of Stomatology 2012;47(z1):123-126
CountryChina
Language:Chinese
Abstract: Objective To determine the effect of berberine on cell cycle of Candida albicans.Methods Flow cytometry (FCM) was used to determine the effect of berberine on cell cycle of Candida albicans.After Candida albicans (ATCC76615) was incubated in yeast extract peptone dextrose (YPD)medium which contained different concentration of berberine for 6 hours,12 hours and 48 hours respectively,the cell cycle was detected by FCM.The proliferation index(PI) was calculated.Results PI was significantly inhibited when Candida albicans (ATCC76615)was incubated with berberine for 12 hours (0 g/L:98.8,0.04 g/L:82.4,0.08 g/L:75.6,0.16 g/L:54.1).Conclusions The berberine could inhibit the cell cycle of Candida albicans in vitro,wbich may be the potential mechanism of antifungal effect of berberine against Candida albicans.