Cellular damage in oral mucosa cells following dental X-ray
10.3760/cma.j.issn.1002-0098.2012.s1.019
- VernacularTitle:口腔X线检查对细胞损伤的探讨
- Author:
Shuai HAO
1
;
Zhi-Ling ZHANG
;
Gang LI
;
Wei HU
;
Bing-Shuang ZOU
;
Xu-Chen MA
Author Information
1. 100081,北京大学口腔医学院·口腔医院口腔颌面放射科
- Keywords:
X-ray;
Mouth mucosa;
Micronucleus test
- From:
Chinese Journal of Stomatology
2012;47(z1):85-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the genetic damage and cellular death in exfoliated buccal mucosa cells from patients undergoing dental Oral and maxillofacial radiology.Methods Orthodontics (14 male and 10 male) before and 7-10 days after they underwent different series of X-ray examinations.Cytological preparations were successively dyed with methods of Feulgen and fast-green,and analyzed under a fluorescence microscope.Micronucleated cells and other type of cells (basal cells,binucleated cells,condensed chromatin cells,karyorrhectic cells,pyknotic cells,karyolytic cells and cells with nuclear buds) were scored in 1000 cells.Results There was no statistically significant difference (P ＞0.05) between the frequency of micronucleated cells before and after dental X-ray exposures.The frequency of micronucleus was not significantly different between the patients aged under 18 and those over 18 years old.Large dose exposure was related to increased karyolytic cells (P =0.03).Conclusions X-ray dose was directly related to increased cell apotosis.The patients younger than 18 years old were not more susceptible to X-ray than those over 18.
