Effect of enamel matrix proteins on proliferation and gene expression of cementum related proteins of human periodontal ligament cells
10.3760/cma.j.issn.1002-0098.2012.s1.006
- VernacularTitle:釉基质蛋白对牙周膜细胞增殖和牙骨质相关蛋白基因表达的影响
- Author:
Yu-Ping QI
1
;
Jing HUANG
;
Wei FENG
;
Mei-Rong WEI
;
Pi-Shan YANG
;
Ai-Mei SONG
Author Information
1. 山东大学口腔医学院牙周科·山东省口腔生物医学重点实验室
- Keywords:
Cell proliferation;
Dental enamel proteins;
Human periodontal ligament cells;
Cementum attachment protein;
Cementum protein-23
- From:
Chinese Journal of Stomatology
2012;47(z1):26-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of enamel matrix proteins (EMP) on proliferation and gene expression of cementum related proteins in human periodontal ligament cells (PDLC).Methods Human PDLC were treated with EMP at concentrations of 0 (control),50,100,200,300 mg/L respectively.At day 1,3,5,7,methyl thiazolyl tetrazolium(MTT) assay was used to measure cell proliferation.At day 7,real-time reverse transcriptase polymerase chain reaction(RT-PCR) was used to detect mRNA expression of cementum attachment protein (CAP) and cementum protein-23 (CP-23).Results EMP significantly increased cell proliferation at concentrations of 50-200 mg/L,but inhibited cell proliferation at concentration of 300 mg/L.The mRNA expression of both CAP and CP-23 at EMP concentrations of 100,200 mg/L (CAP:4.661 ± 0.154,5.923 ± 0.788 ; CP-23:1.222 ± 0.089,3.795 ± 0.640) was significantly higher thanthat of the control (CAP:1.006±0.062,CP-23:1.012±0.163),P＜0.05.Conclusions EMP can promote cell proliferation and increase the gene expression of CAP and CP-23.It suggests that EMP may enhance cementum regeneration through promoting human PDLC's proliferation and cementum related protein production.
