Ex vivo biological properties of Ceramicrete-based root-end filling materials
10.3760/cma.j.issn.1002-0098.2011.s1.020
- VernacularTitle:Ceramicrete根管倒充填材料的生物学性能评估
- Author:
Sui MAI
1
;
Li-Sha GU
;
R.Tay FRANKLIN
;
Jun-Qi LING
Author Information
1. 中山大学光华口腔医学院
- Keywords:
Root canal obturation;
Cytotoxicity tests,immunologic;
Ceramicrete;
Bioactivity
- From:
Chinese Journal of Stomatology
2011;46(z1):84-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective The ex vivo cytotoxicity of Ceramicrete-based root-end filling materials were tested to lay the experimental foundation for clinical application.Methods The mitochondrial succinate dehydrogenase activities of a rat osteoblast-like cell line ( Ros 17/2.8 ) to ceramicrete-based root-end filling materials were examined using methyl thiazolyl tetrazolium(MTT) assay.Ultrastructural change of cells was observed under Transmission electron microscopy( TEM ).Set Ceramicrete-based materials immersed in simulated body fluid (SBF) for 24 h were examined with scanning electron microscopy ( SEM ).Results Ceramicrete-based root-end filling materials exhibited severe toxicity in the first stage,relative cell proliferation rates ranged from 0.95% to 2.45%.After which toxicity decreased rapidly over time,mild toxicity was exhibited in the second stage with cell proliferation rates ranging from 73.65% -88.62%.Nontoxicity was achieved in the sixth stage with cell proliferation rates ranging from 91.53% -97.25%.TEM of cells exposed to the Ceramicretes for different time-periods revealed variable degrees of cell injury that were consistent with the MTT results.SEM of the set Ceramicrete-based materials immersed in SBF revealed the presence of needle-shaped crystallites over the material surfaces.Conclusions The experimental Ceramicrete-based materials exhibited good biocompatibility and potential bioactivity.