Receptor activator of nuclear factor-κB ligand expression in endothelial cell co-cultured with oral squamous cell carcinoma
10.3760/ema.j.issn.1002-0098.2011.s1.012
- VernacularTitle:共培养体系下血管内皮细胞中破骨细胞相关因子的表达
- Author:
Nian-Hui CUI
1
;
Wei ZHANG
;
En-Bo WANG
Author Information
1. 北京大学口腔医学院(口腔医院)
- Keywords:
Carcinoma,squamous cell;
Osteoclasts;
Vascular endothelial cell
- From:
Chinese Journal of Stomatology
2011;46(z1):50-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of osteoclast-related cytokines in the endothelium cell under co-culture with oral squamous cell carcinoma(OSCC).Methods Three pairs of cell lines were co-cultured in a transwell membrane-separated wells.They were EOMA and SCC Ⅶ,cell lines from mouse; Tca8113 and HUVEC-2,cell lines from human; cells digested from the specimen of OSCC and umbilic vein.The expression of receptor activator of nuclear factor-κB ligand(RANKL) in endothelium cells was examined by immnohistochemistry before and after co-culture.Results There were RANKL positive endothelium cells after co-culure with OSCC in all the three pairs of co-culture system.Conclusions The OSCC cells could induce endothelium cells to express osteoclast related cytokines that would promote the differentiation and activation of osteoclast.This might be part of the mechanism of bone invasion in OSCC patients.
