Effect of recombinant human interleukin-1β on the proteomics of dental pulp cell

VernacularTitle:重组人白细胞介素1β对人牙髓细胞蛋白质组的影响
Author: Shi-Liang GUO ¹ ; Ying-Jie ZHANG ; Yang HUANG ; Ying-Li ZHANG ; Xiang-E BAI
Author Information

1. 吉林大学口腔医学院
Keywords: Interleukin-1 β; Proteome; Human dental pulp cells
From: Chinese Journal of Stomatology 2011;46(z1):15-18
CountryChina
Language:Chinese
Abstract: Objective To compare the similarities and differences of the dental pulp cells in protein expression under different conditions and to investigate the effect of recombinant human interleukin-1β ( rhIL -1 β) on the production of protein by human dental pulp cells (HDPC) in vitro.Methods HDPC were induced with rhIL-1 β for 12 hours and the dental pulp cell entire protein was separated by a 2-DE technique.The rhIL-1β induction and the normal dental pulp cell protein 2-DE atlas were established.Difference expression protein was confirmed by Image Master 2D Elite 5.0 software analysis.Results In induced dental pulp cells,39 protein spots were obviously different,of which 15 points were up-regulated,13 were new protein spots,7 points were down-regulated,4 points expressed only in the control group.Conclusions Two-dimensional gel electrophoresis can not only show the whole protein of human dental pulp cells are likely to be involved in the process of dental pulp cell damage.