Alteration of Fas/FasL gene expression in oral squamous cell carcinoma cell line Tca83 by retinoid acid
10.3760/j.issn:1002-0098.2001.03.017
- VernacularTitle:维甲酸对人舌鳞癌细胞株Tca83 Fas/FasL基因表达的影响
- Author:
Weiqun GUAN
1
;
Shifeng YU
;
Yan GAO
Author Information
1. Affiliated Union Hospital Fujian Medical University
- From:
Chinese Journal of Stomatology
2001;36(3):215-218
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the induction of apoptosis by retinoid acid(RA) in Tca83 cell line and the expression of correlative Fas/FasL gene. Methods Apoptosis of Tca83 was detected by TUNEL, the expression of Fas/FasL-mRNA was detected by RT-PCR and cell immunohistochemical technique. Results The cell apoptosis number of Tca83 in RA group was significantly higher than that in non-RA groupe on day 5(P<0.05). The exepression of Fas-mRNA in RA group was also much higher than that in non-RA group on day 5 (P<0.05), but the level of FasLmRNR had no change. Immunohistochemistry also showed, after RA treatment, the Fas stain was enhanced. Conclusion The result suggests that apoptosis induced by RA may be resulted from the enhancement of Fas gene transcription and translation.
