The preliminary application of mNGS in the diagnosis of invasive fungal sinusitis
10.3760/cma.j.cn115330-20231129-00253
- VernacularTitle:mNGS在侵袭性真菌性鼻窦炎诊断中的初步应用
- Author:
Chenting ZHANG
1
;
Yibo LIANG
;
Jingtai ZHI
;
Liang ZHAO
;
Peng LIN
;
Wei WANG
;
Guimin ZHANG
Author Information
1. 天津市第一中心医院耳鼻咽喉头颈外科 天津市耳鼻喉科研究所 天津市医学重点学科（耳鼻咽喉科学）天津市耳鼻喉质量控制中心，天津 300192
- Keywords:
Sinusitis;
Fungi;
Metagenomics next generation sequencing;
Diagnosis
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2024;59(5):464-471
- CountryChina
- Language:Chinese
-
Abstract:
Objective:By conducting a retrospective analysis of the clinical data of 14 patients diagnosed with invasive fungal rhinosinusitis (IFRS) confirmed by metagenomics next generation sequencing (mNGS) technology, we aim to explore the rapid diagnosis value of mNGS in IFRS.Methods:The clinical data of 14 IFRS patients admitted to TianJin First Central Hospital were retrospectively analyzed from February 2021 to October 2023. The study cohort comprised 8 males and 6 females, with ages ranging from 14 to 77 years. All patients were diagnosed as IFRS by performing mNGS sequencing technology of nasal sinus lesion biopsy specimens. Clinical data such as laboratory examination, imaging examination, histopathological examination results, treatment plan and prognosis were summarized and analyzed.Results:All 14 patients were diagnosed as IFRS, with mNGS detecting pathogens such as Rhizopus (7 cases), Aspergillus (5 cases), Trichoderma (1 case), and Scedosporium apiospermum (1 case). Follow-up evaluations were conducted for a period ranging from 2 months to 2 years post-treatment. At the end of follow-up, 11 out of 14 IFRS patients achieved a complete cure with no signs of recurrence, while the symptoms of the remaining 3 patients significantly improved with comprehensive treatment. Conclusion:mNGS emerges as a highly effective diagnostic tool for IFRS, providing valuable microbiological evidence for clinical diagnosis and demonstrating promising clinical utility.
