The expression profile and potential regulatory mechanism of ACE2 in chronic rhinosinusitis with nasal polyps
10.3760/cma.j.cn115330-20210731-00504
- VernacularTitle:ACE2在慢性鼻窦炎伴鼻息肉中的表达及调控分析
- Author:
Weiqiang HUANG
1
;
Zizhen HUANG
;
Xiaoping LAI
;
Yue LI
;
Xiaohong CHEN
;
Haotian WU
;
Lihong CHANG
;
Yana ZHANG
;
Gehua ZHANG
Author Information
1. 中山大学附属第三医院耳鼻咽喉头颈外科，广州 510630
- Keywords:
ACE2;
ECRSwNP;
non-ECRSwNP;
IFN-γ;
IL-5;
IL-13;
miRNA;
Macrophage
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2022;57(6):692-698
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To preliminarily analyze the expression of angiotensin-converting enzyme 2 (ACE2) and to investigate its potential regulatory mechanism in chronic rhinosinusitis with nasal polyps (CRSwNP).Methods:Patients underwent nasal endoscopic surgery in the Third Affiliated Hospital of Sun Yat-sen University from February 2020 to May 2021 were selected, including 17 males and 6 females, aging from 23 to 66 years old. Expression of ACE2 was evaluated via immunohistochemical staining in controls with non-chronic rhinosinusitis, non-eosinophilic CRSwNP (non-ECRSwNP), and eosinophilic CRSwNP (ECRSwNP) tissue, respectively. Correlations between ACE2 and the indicated Th1/Th2-related cytokines (IFN-γ, IL-4, IL-5, IL-13, IL-25, IL-33, TSLP and periostin) were analyzed based on GSE72713 dataset. Protein-protein interaction (PPI) network was constructed via string database, immune infiltration of GSE72713 dataset was evaluated using cibersort algorithm. ACE2 was comprehensively analyzed by microRNA regulatory network, gene set enrichment analysis (GSEA) and pharmacological analysis. Statistical analysis was performed using GraphPad 7.0 and SPSS 20.0 software.Results:ACE2 was up-regulated in non-ECRSwNP compared with ECRSwNP. Microarray analysis showed that ACE2 was positively correlated with IFN-γ while inversely correlated with IL-5, IL-13 and periostin significantly. Analysis of immune infiltration suggested that ACE2 expression correlated positively with the number of M1 macrophage while negatively with M2 macrophage. GSEA demonstrated that interferon-related signaling pathways were up-regulated in non-ECRSwNP, and miRNA-200B/miRNA-200C/miRNA-429 pathways targeting ACE2 were enriched in ECRSwNP. Results of pharmacological analysis indicated that ampicillin was able to promote the expression of ACE2 whereas acetaminophen could down regulated the expression of ACE2.Conclusion:Expression pattern of ACE2 is varied in non-ECRSwNP and ECRSwNP, which may be related to the different infiltration of indicated cytokines and different regulatory pathways of miRNA.
