Expressions of angiogenesis-related factors: CD105, EphA2 and EphrinA1 in laryngeal squamous cell carcinoma and clinical implication
10.3760/cma.j.issn.1673-0860.2016.12.010
- VernacularTitle:肿瘤血管生成相关因子CD105、 EphA2及其配体EphrinA1在喉鳞状细胞癌中的表达及意义
- Author:
Juan SU
1
;
Xiaobin JI
;
Jinghua XIE
;
Wen LI
Author Information
1. 510180,广州市第一人民医院耳鼻咽喉科
- Keywords:
Laryngeal Neoplasms;
Carcinoma,squamous Cell;
Immunohistochemistry;
Neovascularization,pathologic;
EphA2;
EphrinA1
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2016;51(12):929-936
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of endoglin (CD105),erythropoietinproducing hepatocyte receptor A2 (EphA2) and its ligand ephrinA1 proteins in laryngeal squamous cell carcinoma (LSCC) and the relationship between their expressions and the clinicopathological factors of LSCC.Methods The expressions of CD105,EphA2 and EphrinA1 proteins were detected with immunohistochemical staining in LSCC in 76 cases and adjacent normal laryngeal tissues (ANLT) (S-P) in 25 cases.SPSS 17.0 software was used to analyze the data.Results The mean microvessel density (MVD) value marked by CD105 staining in LSCC was 10.33 ± 2.29,which was significantly higher than that in ANLT(1.20 ± 1.04,t =18.732,P ＜ 0.05).The CD105-MVD was correlated with T stage,histological grading,clinical stage,lymph node metastasis,recurrence and prognosis in LSCC (F value was 5.34,4.79,5.36,t value was-2.70,2.56,all P ＜ 0.05).The positive expression rates of EphA2 and EphrinA1 in LSCC were 78.95％ (60/76),and 81.85％ (62/76),which were respectively significantly higher than 40％ (10/25) for EphA2 expression and 44％ (11/25) for EphrinA1,expression in ANLT (x2 value was 13.41,13.26,both P ＜ 0.05).EphA2 expression was correlated with histological grading,T stage,clinical stage,lymph node metastasis,recurrence and prognosis in LSCC (x2 value was 6.25,14.60,15.11,8.52,5.54,all P ＜ 0.05).EphrinA1 expression was correlated with T stage,clinical stage,lymph node metastasis,recurrence and prognosis in LSCC (x2 value was 6.44,12.28,16.78,6.44,all P ＜ 0.05).The expressions of CD105,EphA2 and EphrinA1 were positively correlated with each other r value was 0.72,0.74,0.64,all P ＜ 0.05.Survival analysis indicated that the expressions of CD105 and EphA2,histological grading,lymph node metastasis,clinical stage and recurrence were independent factors for tumor prognosis in LSCC (P ＜ 0.05).Conclusions The expressions of CD105,EphA2 and EphrinA1 protein were positively correlated with each other in LSCC.They may play important roles in the tumorigenesis,malignant progression and poor prognosis of LSCC.
