A murine model of local allergic rhinitis
10.3760/cma.j.issn.1673-0860.2016.07.010
- VernacularTitle:局部变应性鼻炎小鼠模型的建立与研究
- Author:
Baiwen CHEN
1
,
2
;
Shenhong QU
;
Min LI
;
Linsong YE
;
Shaojie ZHANG
;
Taijie QIN
;
Hua FAN
Author Information
1. 530001南宁,广西中医药大学研究生院
2. 530021南宁,广西壮族自治区人民医院耳鼻咽喉科
- Keywords:
Rhinitis,allergic,perennial;
Local;
Interleukin-5;
Interleukin-13
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2016;51(7):533-537
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the murine models of local allergic rhinitis (LAR) and allergic rhinitis (AR) by using ovalbumin (OVA),and to investigate the relationship between them.Methods Thirty BALB/c mice were divided into 5 groups,(1) the nasally sensitized group (group A1) that was challenged with OVA by a 10 d procedure,(2) the control group of A1 that was challenged with phosphatebuffered saline (PBS),(3) the nasally sensitized group (group A2) that was challenged with OVA by a 25 d procedure,(4) the control group of A2 that was challenged with PBS,(5) the intraperitoneally sensitized group (group B).The numbers of sneezing after final challenge were counted,and the serum OVA-specific immunoglobulin E (OVA-slgE),interleukin (IL)-4,IL-13,IL-5 levels in nasal lavage fluid were measured by ELISA.Hematoxylin-eosin staining was performed to evaluate the histological change of nose and lung tissues.Graph Pad Prism 6 software was used to analyze the data.Results Nasally sensitized group A1 displayed LAR symptoms of sneezing and eosinophilic infiltrating,but without increased OVA-sIgE in serum on day 10 compared with the control group of A1(t =0.697,P ＞ 0.05),OVA-sIgE in serum of group A2(2.710 ± 1.406)ng/ml reached to statistical significance and with airway remodeling on day 25 compared with the control group of A2 ((0.221 ± 0.080) ng/ml,t =4.329,P ＜ 0.05).IL-5 and IL-13 in nasal fluid showed a significant increase in the nasally sensitized group A1,compared with the group A2 (t values were 2.442,2.804,P values were less then 0.05).Conclusions A short time intranasal instillation with OVA could establish LAR murine model,continuing OVA challenge could increase serum sIgE level and with airway remodeling.LAR mice show a unique characteristic by expressing higher IL-5 and IL-13 in nose than AR mice,but sIgE in serum remains at a normal level.
