A preliminary analysis on potentially targeted genes of induced chemotherapy in supraglottic laryngeal squamous cell carcinoma
10.3760/cma.j.issn.1673-0860.2016.07.005
- VernacularTitle:声门上型喉鳞状细胞癌诱导化疗潜在靶向基因的初步分析
- Author:
Qian SHI
1
;
Meng LIAN
;
Jugao FANG
;
Honggang LIU
;
Lingzhao MENG
;
Hongzhi MA
;
Ling FENG
Author Information
1. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科耳鼻咽喉头颈科学教育部重点实验室(首都医科大学)北京市头颈部分子病理重点实验室
- Keywords:
Laryngeal neoplasms;
Carcinoma,squamous cell;
Gene expression profiling;
Drug therapy;
Bioinformatics
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2016;51(7):504-510
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the differentially expressed genes produced by paclitaxel,cisplatin and 5-fluorouracil(TPF) regimen induction chemotherapy and the potentially functionally targeted genes of the induc ed chemotherapy in supraglottic laryngeal squamous cell carcinoma.Methods A total of 11 tissue samples from patients diagnosed as supraglottic carcinoma who didnt receive any treatment before were analyzed with microarray.The patients were divided into two groups based on their responses to the induction chemotherapy:7 were sensitive to chemotherapy and 4 were non-sensitive.Gene expressions were detected by Illumina Human HT-12 BeadChip.The bioinformatics analysis online was used to analyze the differentially expressed genes.Results A total of 1 554 differentially expressed genes related to chemosensitivity were found.Analyzed with GO database,the up-regulated genes included the functional sets of biological adhesion,immune system development and stem cell proliferation,and the down-regulated genes included the functional sets of cell junction organization,phosphorus metabolic process and cell morphogenesis involved in differentiation.Analyzed with KEGG database,the up-regulated pathways included p53,cell adhesion and Ras signaling pathways,and the down-regulated pathways included focal adhesion,endocytosis and ErbB signaling pathways.There were statistically significant differences in the expressions of MAPK10,PIK3R5 and JUN genes,which had biological significance,between sensitive patients and non-sensitive patients.Conclusion MAPK10,PIK3R5 and JUN may be considered as potentially functional genes of the induced chemotherapy in supraglottic carcinoma.
