Expression and clinical significance of autophagy-related gene Beclin1 and P62 in nasal polyps
10.3760/cma.j.issn.1673-0860.2016.06.006
- VernacularTitle:自噬相关基因Beclin1及P62在鼻息肉中的表达及临床意义
- Author:
Junjun QI
1
;
Xuefeng HAN
;
Xiaolan CAI
;
Xuezhong LI
;
Liqiang ZHANG
;
Xin FENG
;
Dayu LIU
Author Information
1. 山东大学齐鲁医院耳鼻咽喉科卫生部耳鼻喉科学重点实验室
- Keywords:
Autophagy;
Beclin1;
P62;
Nasal polyps;
Inflammation
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2016;51(6):428-432
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of autophagy-related gene Beclinl and P62 in nasal polyps and its relationship with the pathogenesis of this disease.Methods The specimens were divided into two groups:nasal polyp tissue (n =50) and normal inferior turbinate mucosa (n =20).The general morphology was detected with hematoxylin-eosin(HE) staining,the expression of Beclin1 and P62 was examined with immunohistochemistry(IHC) and real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR).SPSS 20.0 software was used to analyze the data.Results Protein level:The expression of Beclinl in nasal polyp tissue was lower than inferior turbinate mucosa (U =-13.36,P ＜0.01),in contrast,P62 in experimental group was higher than control group(U =12.99,P ＜0.01).mRNA level:The relative quantity of Beclinl and LC3B expressions in nasal polyp were 0.46 ±0.17and 0.46 ± 0.11,which was lower than those in turbinate mucosa 1.11 ± 0.47 and 0.96 ± 0.25.The differences were significant(t value was-4.61,-4.61,both P ＜0.01).But the relative quantity of P62 expression in nasal polyp was 2.19 ± 0.44,which was higher than that in turbinate mucosa (1.05 ± 0.33).The difference was all significant (t =6.16,P ＜ 0.01).Conclusions Compared with control group,the expression of Beclin1 was deficient and P62 was much more.Autophagy was deficient in nasal polyps,which might be in connection with the pathogenesis of the disease.
