Effect of obstructive sleep apnea hypoxia on learning memory capacity after cerebral ischemia-reperfusion in rats
10.3760/cma.j.issn.1673-0860.2016.04.008
- VernacularTitle:阻塞性睡眠呼吸暂停低通气综合征模式低氧对脑缺血再灌注大鼠学习记忆的影响
- Author:
Xiangfei GUO
1
;
Yaning ZHAO
;
Jianmin LI
;
Changxiang CHEN
;
Shuxing LI
Author Information
1. 华北理工大学护理与康复学院
- Keywords:
Sleep apnea,obstructive;
Hypoxia,brain;
Reperfusion injury;
Hippocampus;
Animal testing alternative
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2016;51(4):282-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of obstructive sleep apnea hypoxia on learning memory capacity in rat after ischemia.Methods Eighty healthy male wister rats were randomly divided into:sham operation group (SO group,n =20),merely ischemia group (I/R group,n =20),and obstructive sleep apnea hypoxia for 7 days ischemia group (IH7 + I/R group,n =20),obstructive sleep apnea hypoxia for 21 days ischemia group (IH21 + I/R group,n =20).Obstructive sleep apnea hypoxia ischemia groups were respectively given obstructive sleep apnea hypoxia for 7 days and 21 days.Ischemia animals were prepared cerebral ischemia-reperfusion model by improved pulsinelli four vessels block (4-VO),the morphological changes of hippocampus nerve cells of rat brain were detected with HE,neuron pathology in hippocampal regin was observed using electron microscope,and learning memory capacity of rats were assessed by the Morris water maze test.Results Compared with the SO group,the I/R group demonstrated shortened escaping latency,increased frequency of crossing the platform in the water maze test,decreased survival rate of neurons,and increased apoptotic cells and ultrastructure damages (P ＜ 0.05).Compared with the I/R group,obstructive sleep apnea hypoxia ischemia groups showed shortened escaping latency,increased frequency of crossing the platform,decreased survival rate of neurons,and increased apoptotic cells and ultrastructure damages (P ＜ 0.05),especially in the IH21 + I/R group (P ＜ 0.05).Conclusions Obstructive sleep apnea hypoxia can increase the damage of learning memory capacity.This damage is related to hippocampus nerve loss and ultrastructure injury from obstructive sleep apnea hypoxia.
