Expression and significance of PTEN and HIF-1αproteins in sinonasal inverted papilloma
10.3760/cma.j.issn.1673-0860.2014.05.010
- VernacularTitle:鼻腔鼻窦内翻性乳头状瘤中PTEN和缺氧诱导因子1α的表达及意义
- Author:
Wen ZHANG
1
;
Shuxin WEN
;
Taoyuan ZHANG
;
Binquan WANG
;
Wei GAO
;
Lijuan LI
Author Information
1. 山西医科大学第一临床医学院耳鼻咽喉头颈外科
- Keywords:
Papilloma,inverted;
PTEN phosphohydrolase;
Hypoxia-inducible factor 1,alpha subunit;
Neoplasm recurrence,local
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2014;(5):399-403
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and clinical significance of phosphatase and tensin homology deleted on ehromosome ten ( PTEN ) and hypoxia-inducible factor 1 alpha ( HIF-1α) in sinonasal inverted papilloma (SNIP) of different pathological grades.Methods Fifty-five paraffin samples from patients with SNIP and a control group of 10 paraffin samples of patients with nomal nasal cavity mucosa (NM) who underwent inferior turbinectomy were consisted in this study.Among the 55 cases of SNIP, 30 cases were without dysplasia subtypes , 11 cases were with dysplasia subtypes , and 14 cases with canceration to squamous cell carcinoma ( SCC ) subtypes.PTEN and HIF-1αexpression in SNIP was detected by immunohistochemistry.The differences between NM and SNIP , and among the three subtypes were analyzed , and the relationship between PTEN , HIF-1αexpression and SNIP recurrence and the correlation between PTEN expression and HIF-1αexpression were also analyzed.SPSS 16.0 software was used to analyze the data.Results The positive expression rate of PTEN in NM and SNIP was 100% and 65.5%, the difference was significant ( U =147, P =0.014), while HIF-1αwas 0 and 30.9%, the difference was significant (U=190, P=0.045).The positive expression rate of PTEN in SNIP without dysplasia, SNIP with dysplasia and NSCC was 83.3%, 63.6%, 28.6%, respectively, the difference was significant(H =12.644, P =0.002); while HIF-1αwas 16.7%, 45.5%, 50.0%, respectively, the difference was significant(H=8.292, P=0.016).A total of 22 SNIP patients recurred.PTEN had lower expression in recurrent SNIP ( 45.5%) than that in non-recurrent SNIP ( 82.8%) , and the difference was significant(χ2 =7.834, P =0.005).However, the expression of HIF-1αhad no significant difference between recurrent SNIP and the SNIP which had no recurrence (χ2 =0.901, P=0.343).The expression of PTEN protein was negatively correlated with that of HIF-1αprotein (r=-0.503, P=0.001).Conclusions PTEN expression decreased graduately with the severity of malignancy of SNIP , but HIF-1αincreased.The expression of HIF-1αwas induced by hypoxia , which may negatively effect the expression of PTEN , and both HIF-1αand PTEN may play critical roles in the progress of SNIP.PTEN is one of the factors responsible for the postoperative recurrence of SNIP.
