Construction of plasma metabolic profile of type 2 diabetic mellitus patients based on UPLC-QTOF/MS non-targeted metabonomics
10.13699/j.cnki.1001-6821.2024.19.026
- VernacularTitle:基于UPLC-QTOF/MS的非靶向代谢组学构建2型糖尿病患者血浆代谢轮廓的研究
- Author:
Man-Li ZHU
1
,
2
;
Yan-Dong FAN
;
Ye WANG
;
Feng-Cai JI
;
Xue YANG
;
Xue-Hui LI
;
Lin-Lin LI
Author Information
1. 新疆医科大学中心实验室,新疆维吾尔自治区乌鲁木齐 830017
2. 新疆医科大学新疆地区高发疾病研究教育部重点实验室,新疆维吾尔自治区乌鲁木齐 830017
- Keywords:
ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry;
type 2 diabetes millitus;
Uygur;
Kazak;
non-targeted metabonomics
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(19):2891-2895
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of plasma metabolites in Uygur and Kazak patients with type 2 diabetes mellitus(T2DM)and people with normal glucose tolerance(NGT),construct the profile of different metabolites and analyze different metabolic pathways.Methods Plasma samples of T2DM and NGT from Uygur and Kazak were collected for detection.Ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry was used for detection.MetaboAnalyst 5.0 was used to analyze the data,and the screening conditions were P＜0.05 and fold change(FC)＜0.90 or FC＞1.10.We used The Kyoto Encyclopedia of Genes and Genomes database to enrich and explore the metabolic pathways of different metabolites.Results Compared with the Uighur NGT group,there are 109 differential metabolites in the T2DM group,and 43 unique differential metabolites are mainly concentrated in five pathways,such as phenylalanine biosynthesis,tyrosine biosynthesis,and tryptophan metabolism.Compared with the NGT group,Kazak T2DM has 86 differential metabolites,and 28 unique differential metabolites are mainly enriched in the urea cycle,arginine,and proline metabolism(impact＞0.10,P＜0.05).Conclusion In this study,the plasma metabolic profile of T2DM patients was constructed,and the unique metabolites of T2DM in Uygur and Kazak were screened out,which provided potential biomarkers for early prevention of T2DM.
